Inhibition of Cell Proliferation and Migration by miR-509-3p That Targets CDK2, Rac1, and PIK3C2A |
| |
Authors: | Sena Yoon Eunji Han Young-Chul Choi Honghwan Kee Yongsu Jeong Jaeseung Yoon Kwanghee Baek |
| |
Institution: | 1.Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Korea |
| |
Abstract: | CDK2 is a key regulator of cell cycle progression. In this study, we screened for miRNAs targeting CDK2 using a luciferase-3′-untranslated region reporter assay. Among 11 hit miRNAs, miR-509-3p reduced CDK2 protein levels and significantly inhibited cancer cell growth. Microarray, Western blotting, and luciferase reporter analyses revealed additional targets of miR-509-3p, including Rac1 and PIK3C2A. Overexpression of miR-509-3p induced G1 cell-cycle arrest and inhibited colony formation and migration. RNAi experiments indicated that the growth-inhibitory effects of miR-509-3p may occur through down-regulation of CDK2, Rac1, and PIK3C2A. Targeting of multiple growth regulatory genes by miR-509-3p may contribute to effective anti-cancer therapy. |
| |
Keywords: | CDK2 growth inhibition microRNA miR-509-3p PIK3C2A Rac1 |
|
|