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Identification of differential genes in the ovary relative to the testis and their expression patterns in half-smooth tongue sole (Cynoglossus semilaevis) |
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| Authors: | Yeying Sun a b Quanqi Zhang a Jie Qi a Yanjie Chen a Qiwang Zhong a Chunmei Li a Yan Yu a Shuo Li a Zhigang Wang a a Key Laboratory of Marine Genetics Breeding Ministry of Education Qingdao China b College of Pharmacy Binzhou Medical University Yantai China |
| Institution: | Yeying Sun a,b,Quanqi Zhang a,Jie Qi a,Yanjie Chen a,Qiwang Zhong a,Chunmei Li a,Yan Yu a,Shuo Li a,Zhigang Wang a a Key Laboratory of Marine Genetics , Breeding (College of Marine Life Science,Ocean University of China),Ministry of Education,Qingdao 266003,China b College of Pharmacy,Binzhou Medical University,Yantai 264003,China |
| Abstract: | Half-smooth tongue sole (Cynoglossus semilaevis) is a rare marine flatfish whose mature ovary and testis greatly differ in volume and weight. The length and weight of mature females are over twice greater than those of mature males. To obtain sufficient information on gonad differentiation and the relationship between gonad development and growth in the fish, we compared gene expression between the ovary and testis using suppression subtractive hybridization (SSH). Testis cDNAs are subtracted from ovary cDN... |
| Keywords: | Cynoglossus semilaevis SSH gene expression sexual dimorphism RT-PCR |
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