Fibrin patch-based insulin-like growth factor-1 gene-modified stem cell transplantation repairs ischemic myocardium |
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| Authors: | Jun Li Kai Zhu Shan Yang Yulin Wang Changfa Guo Kanhua Yin Chunsheng Wang Hao Lai |
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| Institution: | 1.Department of Cardiac Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, P.R. China;2.Shanghai Institute of Cardiovascular Disease, Shanghai 200032, P.R. China;3.Department of Radiology, Zhongshan Hospital, Fudan University, Shanghai 200032, P.R. China |
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| Abstract: | Bone marrow mesenchymal stem cells (BMSCs), tissue-engineered cardiac patch, and therapeutic gene have all been proposed as promising therapy strategies for cardiac repair after myocardial infarction. In our study, BMSCs were modified with insulin-like growth factor-1 (IGF-1) gene, loaded into a fibrin patch, and then transplanted into a porcine model of ischemia/reperfusion (I/R) myocardium injury. The results demonstrated that IGF-1 gene overexpression could promote proliferation of endothelial cells and cardiomyocyte-like differentiation of BMSCs in vitro. Four weeks after transplantation of fibrin patch loaded with gene-modified BMSCs, IGF-1 overexpression could successfully promote angiogenesis, inhibit remodeling, increase grafted cell survival and reduce apoptosis. In conclusion, the integrated strategy, which combined fibrin patch with IGF-1 gene modified BMSCs, could promote the histological cardiac repair for a clinically relevant porcine model of I/R myocardium injury. |
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| Keywords: | Insulin-like growth factor-1 mesenchymal stem cells fibrin patch myocardial infarction cardiac repair |
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