Salt-stress induced changes in the leaf proteome of diploid and tetraploid mandarins with contrasting Na and Cl accumulation behaviour |
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Authors: | Alessandra Podda Giovanni Checcucci Wafa Mouhaya Delphine Centeno Valerie Rofidal Renata Del Carratore François Luro Raphael Morillon Patrick Ollitrault Bianca Elena Maserti |
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Institution: | 1. CNR-IBF, Istituto di BioFisica, Area della Ricerca, Via Moruzzi 1, I-56124 Pisa, Italy;2. CIRAD-UPR, Amélioration Génétique D‘espèces à Multiplication Vegetative, Avenue Agropolis, 34398 Montpellier, Cedex 5, France;3. INRA-UR 1199 Protéomique Fonctionnelle, Place Pierre Viala, 34060 Montpellier, Cedex 2, France;4. CNR-IFC, Istituto di Fisiologia Clinica, Area della Ricerca, Via Moruzzi 1, I-56124 Pisa, Italy;5. INRA-UR GEQUA, San Giuliano, F-20230, San Nicolao, Corse, France |
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Abstract: | To understand the genotypic variation of citrus to mild salt stress, a proteomic approach has been carried out in parallel on two citrus genotypes (‘Cleopatra’ and ‘Willow leaf’ mandarins), which differ for Na+ and Cl− accumulation, and their cognate autotetraploids (4×). Using two-dimensional electrophoresis approximately 910 protein spots were reproducibly detected in control and salt-stressed leaves of all genotypes. Among them, 44 protein spots showing significant variations at least in one genotype were subjected to mass spectrometry analysis for identification. Salt-responsive proteins were involved in several functions, including photosynthetic processes, ROS scavenging, stress defence, and signalling. Genotype factors affect the salt-responsive pattern, especially that of carbon metabolism. The no ion accumulator ‘Cleopatra’ mandarin genotype showed the highest number of salt-responsive proteins, and up-regulation of Calvin cycle-related proteins. Conversely the ion accumulator ‘Willow leaf’ mandarin showed high levels of several photorespiration-related enzymes. A common set of proteins (twelve spots) displayed higher levels in salt-stressed leaves of 2× and 4× ‘Cleopatra’ and 4× ‘Willow leaf’ mandarin. Interestingly, antioxidant enzymes and heat shock proteins showed higher constitutive levels in 4× ‘Cleopatra’ mandarin and 4× ‘Willow leaf’ mandarin compared with the cognate 2× genotype. This work provides for the first time information on the effect of 8 weeks of salt stress on citrus genotypes contrasting for ion accumulation and their cognate autotetraploids. Results underline that genetic factors have a predominant effect on the salt response, although a common stress response independent from genotype was also found. |
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Keywords: | Citrus Citrus reshni Citrus deliciosa Leaf proteome Polyploidy Salt stress |
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