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β-N-Acetylhexosaminidase involvement in α-conglutin mobilization in Lupinus albus |
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| Authors: | Cláudia N Santos Marta Alves António Oliveira Ricardo B Ferreira |
| Institution: | 1. Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Apartado 127, 2781-901 Oeiras, Portugal;2. Instituto de Biologia Experimental e Tecnológica, Apartado 12, 2781-901 Oeiras, Portugal;3. Instituto Superior de Agronomia, Universidade Técnica de Lisboa, 1349-017 Lisboa, Portugal |
| Abstract: | Glycosylation is an important post-translational modification involved in the modulation of a wide variety of cellular processes. Because glycosydases are central, the aim of this study was to investigate the glycosyl activity present in the cotyledons of the seeds of an important crop legume, Lupinus albus, as well as potential natural substrates of the detected enzymes. |
| Keywords: | DMS dimethylsuberimidate DW dry weight FW fresh weight β-ME β-mercaptoethanol GalNAc N-acetylgalactosamine GlcNAc N-acetylglucosamine β-NAHase β-N-acetylhexosaminidase pNP p-nitrophenyl pNP&ndash GalNAc p-nitrophenyl&ndash β-d-N-acetylgalactosamine pNP&ndash GlcNAc p-nitrophenyl&ndash β-d-N-acetylglucosamine pNP&ndash (GlcNAc)2 p-nitrophenyl&ndash β-d-N N&prime -diacetylchitobiose pNP&ndash (GlcNAc)3 p-nitrophenyl&ndash β-d-N N&prime N&Prime -triacetylchitotriose pNP&ndash Glcp p-nitrophenyl&ndash α-d-glucopyranosyde |
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