Rodent-Borne Hantaviruses in Cambodia, Lao PDR, and Thailand |
| |
Authors: | Kim Blasdell Jean Fran?ois Cosson Yannick Chaval Vincent Herbreteau Bounneuang Douangboupha Sathaporn Jittapalapong Ake Lundqvist Jean-Pierre Hugot Serge Morand Philippe Buchy |
| |
Institution: | Virology Unit, Institut Pasteur du Cambodge, Phnom Penh, Cambodia. |
| |
Abstract: | In order to evaluate the circulation of hantaviruses present in southeast Asia, a large scale survey of small mammal species was carried out at seven main sites in the region (Cambodia, Lao People's Democratic Republic, and Thailand). Small scale opportunistic trapping was also performed at an eighth site (Cambodia). Using a standard IFA test, IgG antibodies reacting to Hantaan virus antigens were detected at six sites. Antibody prevalence at each site varied from 0 to 5.6% with antibodies detected in several rodent species (Bandicota indica, B. savilei, Maxomys surifer, Mus caroli, M. cookii, Rattus exulans, R. nitidius, R. norvegicus, and R. tanezumi). When site seroprevalence was compared with site species richness, seropositive animals were found more frequently at sites with lower species richness. In order to confirm which hantavirus species were present, a subset of samples was also subjected to RT-PCR. Hantaviral RNA was detected at a single site from each country. Sequencing confirmed the presence of two hantavirus species, Thailand and Seoul viruses, including one sample (from Lao PDR) representing a highly divergent strain of Seoul virus. This is the first molecular evidence of hantavirus in Lao PDR and the first reported L segment sequence data for Thailand virus. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|