Enhanced salinity tolerance and improved yield properties in Bangladeshi rice Binnatoa through <Emphasis Type="Italic">Agrobacterium-</Emphasis>mediated transformation of <Emphasis Type="Italic">PgNHX1</Emphasis> from <Emphasis Type="Italic">Pennisetum glaucum</Emphasis> |
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Authors: | S M Touhidul Islam R S Tammi Sneh L Singla-Pareek Zeba Islam Seraj |
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Institution: | (1) Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, Bangladesh;(2) Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Bangladesh;(3) International Centre for Genetic Engineering and Biotechnology, New Delhi, India; |
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Abstract: | Rice yield is severely affected by high-salt concentration in the vicinity of the plant. In an effort to engineer rice for
improved salt tolerance Agrobacterium-mediated transformation of rice cv. Binnatoa was accomplished with the Pennisetum glaucum vacuolar Na+/H+ antiporter gene (PgNHX1) under the constitutive CaMV35S promoter. For the molecular analysis of putative transgenic plants, PCR and RT-PCR were performed.
Transgenic rice plants expressing PgNHX1 showed better physiological status and completed their life cycle by setting flowers and seeds in salt stress, while wild-type
plants exhibited rapid chlorosis and growth inhibition. Moreover, transgenic rice plants produced higher grain yields than
wild-type plants under salt stress. Assessment of the salinity tolerance of the transgenic plants at seedling and reproductive
stages demonstrated the potential of PgNHX1 for imparting enhanced salt tolerance capabilities and improved yield. |
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