Plant regeneration from the protoplasts of Solanum tuberosum, S. nigrum and S. bulbocastanum |
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Authors: | Anna Szczerbakowa Maria Borkowska Bernard Wielgat |
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Institution: | (1) Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5A, 02-106 Warsaw, Poland |
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Abstract: | The mesophyll protoplasts were isolated from the Solanum tuberosum (S. tbr) clones of different ploidy level (4x Bzura cv., 2x H-8105, and 2x ZEL-1136) as well as from the wild species: S. bulbocastanum (S. blb, 2x) and two accessions of S. nigrum (S. ngr, 6x). Additionally, the protoplasts were isolated from the cell suspensions of Bzura cv. and H-8105 clone. The conditions
of protoplast isolation as well as the media for their culturing and regeneration, were selected and optimized for the studied
genotypes. For mesophyll protoplasts, the shooting calli were produced by all the cultured protoclones except that of S. bulbocastanum. The shoots excised from the protoplast-derived calli developed into whole plants in all the studied potato clones but only
in one accession of S. nigrum, i.e. S. ngr var. gigantea. As for suspension-cell-derived protoplasts, only H-8105 clone produced the regenerative type of calli, though normal shoots
could not be obtained. The regenerative capacity of the protoplasts isolated from leaves and cell suspensions is compared
and discussed.
We regret to report the death of M. Sc. Maria Borkowska after the completion of this work. |
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Keywords: | protoplast regeneration Solanum bulbocastanum Solanum nigrum Solanum tuberosum |
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