Drought stress effects on Rubisco in wheat: changes in the Rubisco large subunit |
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Authors: | Klimentina Demirevska Diana Zasheva Rumen Dimitrov Lyudmila Simova-Stoilova Maria Stamenova Urs Feller |
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Institution: | 1. Institute of Plant Physiology, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bdg. 21, 1113, Sofia, Bulgaria 2. Institute of Biology and Immunology of Reproduction, Bulgarian Academy of Sciences, 1113, Sofia, Bulgaria 3. Institute of Plant Sciences, University of Bern, 3013, Bern, Switzerland
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Abstract: | The leaf protein pattern from drought-tolerant and drought-sensitive wheat varieties subjected to severe soil drought but with the possibility for recover from stress was studied by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). The spots representing Rubisco large subunit (RLS) were identified using polyclonal antibodies against Rubisco and immunoblotting. Some qualitative and quantitative differences in the 2D-PAGE protein map of wheat varieties were revealed under drought conditions. Three days recovery of wheat plants were not enough for restoring RLS quantity to the level of controls after 7 days drought, especially in the drought-sensitive variety Miziya. There are contradictory data in the literature concerning increased or diminished RLS level in drought stressed plants. A comparison of RLS after SDS-PAGE and 2D-PAGE was made. The revealed protein pattern depended on the presence or absence of protease inhibitors in the extraction buffer, on the procedure of extraction, and on the degree of stress. |
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