The histological analysis of indirect somatic embryogenesis on <Emphasis Type="Italic">Drosera spathulata</Emphasis> Labill |
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| Authors: | M Bobák J ?amaj A Pre ová A Blehová E Hlinková M Ove ka A Hlava ka Z Kutar ová |
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| Institution: | (1) Department of Plant Physiology, Comenius University, Mlynská dolina B-2, SK-842 15 Bratislava, Slovak Republic;(2) Institute of Plant Genetics, Slovak Academy of Sciences, Akademická 2, P.O. Box 39 A, SK-950 07 Nitra, Slovak republic;(3) Department of Genetics, Comenius University, Mlynská dolina B-1, SK-842 15 Bratislava, Slovak Republic;(4) Institute of Botany, Slovak Academy of Sciences, Dúbravská cesta 14, SK-842 23 Bratislava, Slovak Republic |
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| Abstract: | We studied indirect somatic embryogenesis in the callus tissue of Drosera spathulata Labill. originated from isolated leaves. Callogenesis was induced on MS medium (Murashige and Skoog 1962), supplemented with
various concentrations of NAA and BA. Somatic embryos regenerated on half-strength MS medium supplemented with 20 μM of NAA
or without growth regulators. The highest efficiency of somatic embryo production was achieved on hormone-free medium. Globular,
heart-, torpedo- and cotyledonary-shaped embryos were observed in embryogenic clusters. Histological and scanning electron
microscopy analysis verifies somatic embryogenesis. Regenerated plants were transferred to soil and were grown to maturity. |
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| Keywords: | Drosera somatic embryogenesis SEM histological studies |
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