Plant regeneration from shoot apical meristems of Melia azedarach L. (Meliaceae) |
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Authors: | Silvia Vila Adriana Scocchi Luis Mroginski |
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Institution: | (1) Instituto de Botánica del Nordeste (IBONE), Facultad de Ciencias Agrarias (UNNE), C.C. 209, 3400 Corrientes, Argentina |
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Abstract: | A protocol was developed for plant regeneration of Melia azedarach L. by in vitro culture of apical meristem (0.5 mm in length). The influence of six clones was investigated. The culture procedure comprised
two sequential steps: 1) Induction of shoots by in vitro culture of axillary buds from adult trees (10–15 years old) by culture on Murashige and Skoog (1962) medium (MS) supplemented
with 0.5 mg·dm−3 BAP (6-benzylaminopurine), 0.1 mg·dm−3 IBA (indolebutyric acid), and 0.1 mg·dm−3 GA3 (gibberellic acid). The Multiplication of the regenerated shoots was achieved in MS + 0.5 mg·dm−3 BAP + 0.1 mg·dm−3 GA3. 2) In vitro culture of the apical meristems from the regenerated shoots in MS medium (0.7 %) supplemented with various combinations of
BAP and IBA.
Maximum shoot proliferation was obtained on MS medium supplemented with 0.5 mg·dm−3 BAP and 0.1 mg·dm−3 IBA.
Regenerated shoots were rooted on MS + 3.5 mg·dm−3 IBA (4 days) followed by subculture on MS lacking growth regulators (30 days). Complete plants were transferred to soil. |
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Keywords: | meristem plant regeneration Melia azedarach genotype forestry |
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