扬子鳄消化道内分泌细胞的免疫组织化学研究

应用７种特异性胃肠激素抗血清对扬子鳄消化道内分泌细胞进行了免疫组织化学定位。５－羟色胺细胞在消化道各段都有分布，以十二指肠密度最高，食道、直肠其次。生长抑素细胞在胃幽门部非常密集，胃体中等，胃贲门部较少，十二指肠偶见。胃泌素细胞主要分布于十二指肠前段，空肠、回肠和直肠偶见。许多血管活性肠肽细胞分布于胃贲门部，胃体和胃幽门部少数。胰高血糖素、胰多肽和Ｐ－物质在消化道各段均未检出阳性细胞。结合扬子鳄的

IMMUNOHISTOCHEMICAL STUDIES ON ENDOCRINE CELLS IN THE DIGESTIVE TRACT OF ALLIGATOR SINENSIS

The distribution and density of the endocrine cells in the digestive tract of Alligator sinensis were studied by immunohistochemical method with seven antisera of gut hormone. 5-Hydroxytryptamine (5-HT)-immunoreactive cells are distributed throughout the digestive tract with the highest density in the duodenum, the second highest in the oesophagus and rectum. Somatostatin-immunoreactive cells occured very numerously in the stomachus Pyloricus, moderately in the stomachus corpus, rarely in the stomachus cardiacus and occasionally in the duodenum. The gastrin-immunoreactive cells were located numerously in the duodenum and occasionally in the jejunum, ileum and rectum.The vasoactive intestinal polypeptide(VIP)-immunoreaetive cells were present mainly in the stomachus cardiacus, rarely in the stomachus corpus and pyloricus. Cells showing immunoreactivity to pancreatic polypeptide, glucagon and substance-P were not detected. Considering the feeding habit, the distributive mode of endocrine cells in the digestive tract of Alligator sinensis was discussed.