| 花背蟾蜍角膜早期形态发生中胶原合成的放射自显影研究 |
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| 引用本文： | 冯伯森,孙颖.花背蟾蜍角膜早期形态发生中胶原合成的放射自显影研究[J].动物学报,1990,36(1):52-57. |
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| 作者姓名： | 冯伯森 孙颖 |
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| 作者单位： | 兰州大学生物学系，兰州大学生物学系，兰州大学生物学系 大连辽宁师范大学生物学系，石家庄河北师范大学生物学系 |
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| 摘 要： | 本实验以~3H-脯氨酸为标记物,用放射自显影方法研究了花背蟾蜍眼的早期发育中胶原的合成、分布以及对角膜早期形态发生的作用。结果表明,角膜上皮从开始形成即合成胶原,并在角膜上皮基底面聚积。在角膜开始透明时,角膜上皮、内角膜和晶状体的胶原合成速率都明显增加,提示与角膜分化密切相关。
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| 关 键 词： | 蟾蜍 角膜 形态发生 胶原 放射显影 |
AUTORADIOGRAPHICAL STUDIES ON THE SYNTHESIS OF COLLAGEN DURING EARLY CORNEAL MORPHOGENESIS IN BUFO RADDEI STRAUCH |
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| FENG BOSEN SUN YING TONG YUNXU.AUTORADIOGRAPHICAL STUDIES ON THE SYNTHESIS OF COLLAGEN DURING EARLY CORNEAL MORPHOGENESIS IN BUFO RADDEI STRAUCH[J].Acta Zoologica Sinica,1990,36(1):52-57. |
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| Authors: | FENG BOSEN SUN YING TONG YUNXU |
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| Abstract: | The present study was designed to investigate the synthesis, distribution and function of collagen during early corneal morphogenesis of Bufo raddei by using 3H-proline labelling and light microscopic autoradiographic technique.Results indicated that collagen was synthesized concomitant with the formation of corneal epithelium and gradually accumulated in the basal surface of corneal epithelium. The synthesis of collagen was increased evidently in corneal epithelium, inner cornea and lens at the beginning of the cornea to become transparent. This result suggested that it might be closely associated with the corneal differentiation. |
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| Keywords: | Bufo raddei Autoradiograph Synthesis of collagen Early corneal morphogenesis |
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