| 草鱼和鲤鱼线粒体 DNA 的分离纯化及其 COI 基因的分子克隆 |
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| 引用本文： | 吴乃虎,王钢锋,阎景智,郭玲,严绍颐,秦鹏春.草鱼和鲤鱼线粒体 DNA 的分离纯化及其 COI 基因的分子克隆[J].动物学报,1991(4). |
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| 作者姓名： | 吴乃虎 王钢锋 阎景智 郭玲 严绍颐 秦鹏春 |
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| 作者单位： | 中国科学院发育生物学研究所 北京
(吴乃虎，王钢锋，阎景智，郭玲，严绍颐)，东北农学院生物工程系 哈尔滨(秦鹏春) |
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| 摘 要： | 本文报道配合使用差速离心和DNaseI核酸酶处理等步骤,从草鱼和鲤鱼新鲜肝组织分离线粒体DNA(mtDNA)的实验方法。这种方法经济简便,纯化的mtDNA产率多,纯度高,经限制性内切酶消化后进行琼脂糖凝胶电泳分离,可以得到清晰的DNA片段谱带,并可直接用于构建酶切图谱和线粒体基因的分子克隆。用这样的mtDNA,我们已克隆了草鱼和鲤鱼的细胞色素氧化酶亚基I基因(COI基因)。
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| 关 键 词： | 线粒体DNA 限制酶图谱 COI基因克隆 |
ISOLATION OF MITOCHONDRIAL DNA AND CLONING OF CYTOCHROME OXIDASE SUBUNIT I GENE FROM GRASS CARP AND CARP~(**) |
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| WU NAI-HU WANG GANG-FENG YAN JING-ZHI GUO LING YAN SHAO-YI.ISOLATION OF MITOCHONDRIAL DNA AND CLONING OF CYTOCHROME OXIDASE SUBUNIT I GENE FROM GRASS CARP AND CARP~(**)[J].Acta Zoologica Sinica,1991(4). |
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| Authors: | WU NAI-HU WANG GANG-FENG YAN JING-ZHI GUO LING YAN SHAO-YI |
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| Abstract: | Here we report a procedure for the isolaion and purification of mitochondrial DNA (mtDNA) from fresh livers of grass carp (Ctenopharyngodon idellus) and carp (Cyprinus carpio L.) by differencial centrifugation and DNaseI treatment. The procedure is economical and convenient. The mtDNA obtained by this procedure showed high purity and high yield. The grass carp mtDNA was digested by several restriction enzyme and clear bands were shown on agarose gel electrophoresis. The purified mtDNA can be used directly for mapping of mtDNA and molecular cloning of mitochondrial genes. We had successfully cloned mitochondrial cytochrome oxidase subunit I (COI) gene from grass carp and carp. |
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| Keywords: | Mitochondrial DNA Restriction mapping COI gene cloning |
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