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| 小鼠白血病抑制因子基因的克隆、表达及其对维持鸡原始生殖细胞未分化状态的影响 | |
| 引用本文： | 杨娜娜,朱靖,史卫峰,孟春花,杜立新.小鼠白血病抑制因子基因的克隆、表达及其对维持鸡原始生殖细胞未分化状态的影响[J].动物学报,2008,54(5). |
| 作者姓名： | 杨娜娜 朱靖 史卫峰 孟春花 杜立新 |
| 作者单位： | 1. 泰山医学生命科学研究所,山东,泰安,271000 2. 山东省实验动物中心,济南,250002 3. 南京农业大学动物科技学院,南京,210095 4. 中国农业科学院畜牧研究所,北京,100094 |
| 基金项目： | 北京市自然科学基金，泰山医学院青年基金 |
| 摘 要： | 为了探索鸡原始生殖细胞(Primordial germ cells,PGCs)适合的培养体系,我们在已构建的分泌型真核表达载体pSecTag-mlif(sp-)的基础上,通过脂质体介导将mlif转染到鸡PGCs和鸡胚胎成纤维(Chicken embryonic fibroblast, CEF)细胞中,48 h后收集细胞上清液,蛋白印迹均检测到小鼠白血病抑制因子(mLIF)的表达.以CEF细胞作饲养层,分七组来培养鸡PGCs,结果发现四组和五组培养的PGCs生长状态最好,三组传代后开始2-3天生长状况较好,3天后克隆周围有明显的分化现象.本实验将已构建了含mLIF基因的分泌型真核表达载体成功地瞬时转染到PGCs和CEF细胞中,且表达的mLIF具有维持鸡PGCs未分化状态的功能. |
| 关 键 词： | 鸡胚胎生殖细胞 转染 脂质体 白血病抑制因子 |
Cloning and expression of mLIF and the effects of mLIF maintaining chicken primordial germ cells in the undifferentiated state |
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| YANG Na-Na,ZHU Jing,SHI Wei-Feng,MENG Chun-Hua,DU Li-Xin.Cloning and expression of mLIF and the effects of mLIF maintaining chicken primordial germ cells in the undifferentiated state[J].Acta Zoologica Sinica,2008,54(5). | |
| Authors: | YANG Na-Na ZHU Jing SHI Wei-Feng MENG Chun-Hua DU Li-Xin |
| Institution: | YANG Na-Na1,ZHU Jing2,SHI Wei-Feng1,MENG Chun-Hua3,DU Li-Xin41.Institute of Life Sciences,Taishan Medicine University,Tai\'an 271000,Shangdong,China2.Sh,ong Provincial Center of Laboratory Animals,Jinan 250002,China3.College of Animal Science , Technology,Nanjing Agriculture University,Nanjing 210095,China4.Institute of Animal Sciences Chinese Academy of Agriculture Sciences,Beijing 100094,China |
| Abstract: | In order to establish the best cultivation system,we constructed eukaryotic expression vector pSecTag-mlif(sp-),transfected chicken PGCs(Primordial germ cells)and CEF(Chicken embryonic fibroblast)cells with pSecTag-mlif(sp-)by the method of lipofectamine mediation,and collected the supernatant after centrifugating the cellular medium.We surveyed mLIF(Mice leukemia inhibitory factor)expression in cellular medium by means of of western-blotting.We cultured PGCs using seven different methods including three co... |
| Keywords: | Chicken embryonic germ cells Rransfect Lipofectamine LIF |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |