| Ca2+对非细胞体系细胞核重建影响机制的研究 |
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| 引用本文： | 赵国燕,张博,翟中和.Ca2+对非细胞体系细胞核重建影响机制的研究[J].动物学报,2000,46(1):95-103. |
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| 作者姓名： | 赵国燕 张博 翟中和 |
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| 作者单位： | 北京大学生命科学学院，北京 100871 |
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| 基金项目： | 国家自然科学基金，39600074， |
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| 摘 要： | 研究Ｃａ＾２＋与非细胞体系细胞核重建的关系以及Ｃａ＾２＋的作用机制，结果显示：Ｃａ＾２＋对非细胞体系中核装配有抑制作用，随着Ｃａ＾２＋浓度的增加，抑制核装配作用增加，在Ｃａ＾２＋作用的早期（作用１０分钟后）中入ＥＧＴＡ，可以部分地逆转Ｃａ＾２＋的抑制作用，Ｃａ＾２＋作用１小时后再加入ＥＧＴＡ，不再有逆转作用。对Ｃａ＾２＋作用机制的研究结果显示，不是由于ＡＴＰ和ＧＴＰ被沉淀而导致细胞核组装被抑制，Ｃ
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| 关 键 词： | Ca^2＋ 非洲爪蟾卵提取物 非细胞体系 核重建 |
STUDIES ON THE MECHANISM OF CALCIUM-INDUCED NUCLEAR RECONSTITUTION IN A CELL-FREE SYSTEM |
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| ZHAO GuoYan,ZHANG Bo,ZHAI ZhongHe.STUDIES ON THE MECHANISM OF CALCIUM-INDUCED NUCLEAR RECONSTITUTION IN A CELL-FREE SYSTEM[J].Acta Zoologica Sinica,2000,46(1):95-103. |
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| Authors: | ZHAO GuoYan ZHANG Bo ZHAI ZhongHe |
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| Abstract: | The relationship between Ca 2+ and nuclear reconstitution in a Ca 2+ free system and the mechanism of Ca 2+ action were studied. The results show that Ca 2+ blocks the cellfree nuclear reconstitution. With the increase of Ca 2+ concentration, the percentage of decondensed chromatin decreases. EGTA is added to stop the action of Ca 2+ after certain times. If EGTA is added 10 min after incubation, nuclear vesicles can attach to the surface of the sperm and form flattened bilayered membrane along its periphery. If EGTA is added 1 hr after incubation, there are only vesicles accumulated along its periphery. No bilayered membrane forms. It seems that this is not because Ca 2+ precipitates ATP and GTP or Ca 2+ activates proteinase or nuclease. CaM is not involved the regulation of nuclear vesicle fusion. Ca 2+ has little effect on the binding process, but blocks the fusion of nuclear vesicles. |
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| Keywords: | Ca~(2+) Xenopus eggs extract Cell-free system Nuclear reconstitution Nuclear membrane |
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