龙洞山溪鲵精子的超微结构

应用透射电镜和扫描电镜对龙洞山溪鲵(Batrachuperuslongdongensis)精子的超微结构进行观察和研究,探讨山溪鲵属以及小鲵科物种精子的结构特征,并探讨有尾两栖类精子结构特征的演化及其与生殖进化的关系。结果表明:1)龙洞山溪鲵的精子具小鲵科物种精子的共同特征,即精子无顶体钩,顶体呈三叶草状,尾部无线粒体,轴纤维粗大呈圆柱状等特征;2)龙洞山溪鲵精子核脊的形态结构与小鲵科其它属物种以及有尾类其它科物种精子的核脊存在明显的差别,由此可见核脊形态结构具有属或种的特异性。此外,与已有报道的有尾两栖类物种精子的结构特征进行分析比较表明:1)有尾类精子特征的分化与其受精方式的变化是一致的;2)精子结构特征支持隐鳃鲵超科为单系的推测

Ultrastructure of the spermatozoa of Longdong stream salamander Batrachuperus longdongensis (Urodela:Hynobiidae)

Ultrastructure of the spermatozoa of longdong stream salamander Batrachuperus longdongensis was studied by both transmission and scanning electron microscopy. The structural features of spermatozoa in Hynobiidae and Batrachuperus, and the relationship between the evolution of sperm and reproductive evolution in urodeles were discussed. The results show that: 1) the spermatozoa of B. longdongensis share synapomorphy with Hynobiidae, e.g. the clover-shaped acrosome without acrosomal barb, absence of mitochondrion in the tail and cylindric axial fibre; 2) the size and structure of nuclear ridge of B. longdongensis differ from other species of Hynobiidae and other families of Urodela significantly, it suggests that the nuclear ridge has specific or generic-specific characteristics in salamanders and newts. Comparative analyses of sperm structures among the tailed amphibians (Urodela) indicates that: 1) the feature evolution of sperm in urodeles is related to the differentiation of their fertilization modes; 2) the characteristics of the sperm structure support for the Cryptobranchoidea monophyly .