脱水休眠小卷蛾斯氏线虫的超微结构

采用扫描电镜，透视电镜和光学显微镜，对在SB高渗液中脱水体眠的小卷蛾斯氏线虫（Steinernema carpocapsae)BJ品系首次进行表态特征观察，结果表明：未脱水处理线虫体表光滑，侧线呈直线状，脱水体眠线虫体躯体稍弯曲，体表出现排列整剂的横向皱纹，侧线弯曲成有规律垢波浪状，脱水体眠后，线虫体长，体宽的明显减小达到了一个稳定值，比未处理线虫分别减少了33．1％和28．4％，脱水休眠线虫皮层和基质层变薄，皮下肌肉层明显变厚，肌肉纤维变形，排列没有方向性，食道与细胞器收缩，单位体积脂肪粒增多，以上这些变化可作为衡量小卷蛾氏线虫进入眠的形态特征参数。

ULTRASTRUCTURE OF STEINERNEMA CARPOCAPSAE BJ IN ANHYDROOUS DORMANCY

SB high osmotic solution is a new type of dehydration agent, which administrated to entomopathogenic nematode Steinernema carpocapsae BJ for 10 hours to make them to be anhydrous and in dormancy. It is the easy, quick and safe way to make nematodes anhydrous and in dormancy. It will be a main proceeding of dehydration to make nematode into dormancy in the future. There are some special morphological characters of anhydrous dormant S. carpocapsae BJ reported in this paper, which are obtained by the observation under the scanning electron microscope, clairvoyant electron microscope and optical microscope. These special morphological characters are the most important parameters of anhydrobiotic nematode for S. carpocapsae, which are helpful to know whether nematodes have already been into dormancy or not, and which can tell the difference from the fresh nematodes. In fact, these morphological characters of anhydrous dormant nematode in the condition of fast dehydration were described for the first time. The anhydrous dormant entomopathogenic nematode S. carpocapsae BJ mainly had 5 aspect characters in morphology. (1) The bodies were bending into slightly curve, on which the transverse wrinkles were regularly in rows and the lateral lines were in the shape of waves. While the bodies of the control were smooth, on which the lateral lines were very straight. (2) The bodies shrank evidently both in length and in width. The percentage of shrinking reached a steady value, which were 33.1% in length and 28.4% in width of the control, respectively. (3) The cortex and matrix got thinner, while the underlying muscles grew thicker. (4) The muscle fibers were in disorderly arrangement. (5) The esophagus and organelle dwindled, while the lipid droplets increased.