| 以ITS1-5.8 SrDNA-ITS2为标记的蓝氏贾第鞭毛虫分子系统发育研究 |
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| 引用本文： | 温少芳,卢思奇,冯宪敏,王凤云.以ITS1-5.8 SrDNA-ITS2为标记的蓝氏贾第鞭毛虫分子系统发育研究[J].动物学报,2006,52(5):954-958. |
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| 作者姓名： | 温少芳 卢思奇 冯宪敏 王凤云 |
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| 作者单位： | 首都医科大学细胞生物学系,北京,100069 |
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| 基金项目： | 国家自然科学基金资助(No.30470243)~~ |
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| 摘 要： | 蓝氏贾第鞭毛虫(Giardia lamblia,又称Gi-ardiaintestinalis或Giardia duodenalis,以下简称贾第虫)是一种广为关注的源真核生物(Archezoa),在生物进化中处于原核生物和真核生物的过渡阶段。在医学上,贾第虫是一种重要的导致腹泻的病原体,其宿主广泛,包括人和大多数脊椎动物。研
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| 关 键 词： | 蓝氏贾第鞭毛虫 核糖体基因内转录间隔区(ITS) 5.8SrDNA 系统发育 |
| 收稿时间： | 2006-04-19 |
| 修稿时间： | 2006-04-192006-07-10 |
Molecular phylogeny of Giardia lamblia based on ITS1-5.8 SrRNA-ITS2 sequence |
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| WEN Shao-Fang,LU Si-Qi,FENG Xian-Min,WANG Feng-Yun.Molecular phylogeny of Giardia lamblia based on ITS1-5.8 SrRNA-ITS2 sequence[J].Acta Zoologica Sinica,2006,52(5):954-958. |
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| Authors: | WEN Shao-Fang LU Si-Qi FENG Xian-Min WANG Feng-Yun |
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| Institution: | Department of Cell Biology, Capital University of Medical Sciences, Beijing 100069 |
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| Abstract: | Giardia lamblia is a widely concerned archezoan. In order to study the phylogeny of G.lamblia, ITS(1 and 2) of ribosome gene(rDNA) and 5.8 SrDNA from various Giardia strains were amplified,cloned and sequenced. A molecular phylogenetic tree was constructed with NJ method. After analyzed by DNAMAN software, 12 bases in 2 of the 10 squences were found to be substituted and 1 of the 2 had one base deletion, while the other 8 squences were quite homologous. The constructed phylogenetic tree was consistent with the phylogenic relationship reported previously. All the results show that ITS(1 and 2) and 5.8 SrRNA genes are effective genetic markers for studies of the evolution of Giardia. |
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| Keywords: | Giardia lamblia Internal transcribed spacer(ITS) of ribosome gene 5 8 SrDNA Phylogeny |
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