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| GenBank数据库中鳜鱼微卫星位点的筛选及特征分析 | |
| 引用本文： | 匡刚桥,刘臻,鲁双庆,刘红玉,张建社,肖调义.GenBank数据库中鳜鱼微卫星位点的筛选及特征分析[J].动物学报,2007,53(1):184-189. |
| 作者姓名： | 匡刚桥 刘臻 鲁双庆 刘红玉 张建社 肖调义 |
| 作者单位： | 1. 长沙大学生物工程与环境科学系,长沙,410003;湖南农业大学动物科技学院,长沙,410128 2. 长沙大学生物工程与环境科学系,长沙,410003 3. 湖南大学环境科学与工程系,长沙,410082 4. 湖南农业大学动物科技学院,长沙,410128 |
| 基金项目： | 国家自然科学基金(30571414)，湖南省自然科学基金(06JJ20056)，湖南省教育厅项目(06C165)~~ |
| 摘 要： | 微卫星(Microsatellite)是一类由2-6个核苷酸经多次单位串联组成的高度变异重复DNA序列(Schlotterer and Tautz,1992)。它具有按照孟德尔方式分离、突变快、多态信息含量丰富、呈共显性遗传等特点,其核心序列在同一物种中具有保守性,因此,可以根据微卫星的侧翼序列设计合适的引 |
| 关 键 词： | 鳜鱼 微卫星 GenBank数据库 |
| 修稿时间： | 2006-07-312006-10-25 |
Isolation and characterization of microsatellite loci of Siniperca chuatsi from GenBank database |
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| KUANG Gang-Qiao,LIU Zhen,LU Shuang-Qing,LIU Hong-Yu,ZHANG Jian-She,XIAO Tiao-Yi.Isolation and characterization of microsatellite loci of Siniperca chuatsi from GenBank database[J].Acta Zoologica Sinica,2007,53(1):184-189. | |
| Authors: | KUANG Gang-Qiao LIU Zhen LU Shuang-Qing LIU Hong-Yu ZHANG Jian-She XIAO Tiao-Yi |
| Institution: | 1. Department of Biotechnology and Environment Science, Changsha University, Changsha 410003, Hunan Province, China;2. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, Hunan Province, China ;3. Department of Environmental Science and Engineering, Hunan University, Changsha 410082, Hunan Province, China |
| Abstract: | This project made use of bioinformatic mining of microsatellites from genomic resources to identify simple sequence repeats(SSRs),or microsatellites.A bioinformatic analysis of 304 nucleotide sequences in Siniperca chuatsi GenBank identified 22 sequences containing 30 microsatellites,account for 9.87% of wholly GenBank database.Cluster analysis indicated that 16 dinucleotide pairs were the most abundant microsatellites,accounting for 53.33% of the total microsatellite-containing sequences;11 trinucleotide repeats and 3 tetranucleotide repeats were found in these microsatellite sequences,accounting for 36.66% and 10.01% respective.17 primer pairs were designed from these microsatellite sequences.Among the 17 primer pairs,13 pairs have amplified products,6 pairs can achieve clear PCR products by Electrophoresis.Genotype analysis results indicated that:the average polymorphism information contents(PIC)were 0.692(ranged:0.567-0.806),were all polymorphic loci. |
| Keywords: | Siniperca chuatsi Microsatellites GenBank database |
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