| 鼠脑突触体Mg^2＋—ATP酶质子泵活性的研究 |
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| 引用本文： | 蔡惠罗,李成勇.鼠脑突触体Mg^2＋—ATP酶质子泵活性的研究[J].动物学报,1995,41(3):299-304. |
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| 作者姓名： | 蔡惠罗 李成勇 |
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| 作者单位： | 中国科学院动物研究所!北京100080 |
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| 摘 要： | 位于突触体质膜的外向型（ｅｃｔｏ）Ｍｇ＾２－ＡＴＰ酶具有水解ＡＴＰ活性，能量偶联的ＡＣ－ＭＡ荧光淬灭实验表明Ｍｇ＾２＋－ＡＴＰ酶水解ＡＴＰ时向膜内转移质子，建立跨膜质子梯度，跨膜质子梯度可以被电中性Ｋ＾＋／Ｈ＾＋离子载体Ｎｉｇｅｒｉｃｉｎ消除，利用Ｈ＾＋敏感的ＢＣＥＣＦ荧光分子测定突触的ｐＨｉ变化，结果表明水解ＡＴＰ产生的质子转移突触体ｐＨｉ下降了光分子测定突触的ｐＨｉ变化，结果表示水解ＡＴＰ产生
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| 关 键 词： | 鼠 脑 突触体 镁 腺苷三磷酸酶 质子泵活性 |
THE PROTON PUMPING ACTIVITY OF Mg~(2+)-ATPase OF Rat Brain Synaptosomes |
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| CAI HUI-LUO LI CHENG-YONG SUN XIAO-LEI.THE PROTON PUMPING ACTIVITY OF Mg~(2+)-ATPase OF Rat Brain Synaptosomes[J].Acta Zoologica Sinica,1995,41(3):299-304. |
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| Authors: | CAI HUI-LUO LI CHENG-YONG SUN XIAO-LEI |
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| Abstract: | The Mg2+-dependent ATPase on the external surface of isolated rat brain synaptosomes has been studied. The rapidquenching of ACMA fluorescence was detected upon hydrolysis of externally added ATP. which could then be restored up-on addition of nigericin. By using the H+-sensitive probe BCECF, a decrease of 0. 11 unit in intrasynaptosomal PH valuewas measured during hydrolysis of ATP. These results indicate that the Mg~2+-ATPase may function as an inward protonpump. P-ch1oromercuribenzoate was found to have inhibitory effect on the proton translocation function. |
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| Keywords: | proton pumping activity synaptosomes Mg~2+-ATPase |
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