二龄草鱼脾脏、肝脏组织高表达甘露糖结合凝集素mRNA

Innate immunity is expected to be very important in fish. Mannose-bingding lectin (MBL) participates in the innate immune system as an activator of the complement system and as an opsonin after binding to certain carbohydrate structures on microorganisms. In this experiment, total mRNA was isolated from spleen, liver, gills, thymus, head kidney and kidney of adult and immature grass carp Ctenopharygodon idllus. The cDNA of MBL was obtained by RT-PCR using total mRNA from the spleen of carp as template. Such cDNA was labled with ^32p and used as probe for Northern analysis, and autoradiographic signals were quantified by densitometry analysis. The results showed that MBL was high expressed in the spleen and liver and low in gills, thymus, head kidney and kidney of adult grass carp, and MBL was much lower expressed in spleen and liver of immature grass carp than those of adult grass carp. The results might partially explain why immature grass carp are vulnerable to grass carp hemorrhage virus (GCHV) whereas adult grass carp are not.This suggested that MBL mav be an imoortant anti-GCHV factor Acta Zoologica Sinica 50 (1): 137 - 140. 2004].

The mannose-binding lectin mRNA is expressed at high level in spleen and liver in adult grass carp

Innate immunity is expected to be very important in fish. Mannose-bing ding lectin (MBL) participates in the innate immune system as an activator of th e complement system and as an opsonin after binding to certain carbohydrate stru ctures on microorganisms. In this experiment, total mRNA was isolated from splee n, liver, gills, thymus, head kidney and kidney of adult and immature grass carp Ctenopharygodon idllus. The cDNA of MBL was obtained by RT-PCR using total mRNA from the spleen of carp as template. Such cDNA was labled with 32 P an d used as probe for Northern analysis, and autoradiographic signals were quantified by den sitometry analysis. The results showed that MBL was high expressed in the spleen and liver and low in gills, thymus, head kidney and kidney of adult grass carp, and MBL was much lower expressed in spleen and liver of immature grass carp tha n those of adult grass carp. The results might partially explain why immature gr ass carp are vulnerable to grass carp hemorrhage virus (GCHV) whereas adult gras s carp are not. This suggested that MBL may be an important anti-GCHV factor .