首页 | 本学科首页   官方微博 | 高级检索  
   检索      

不同发育阶段的斑腿树蛙蝌蚪对敌敌畏遗传毒性的敏感性
引用本文:耿宝荣,薛清清,连迎,姚丹.不同发育阶段的斑腿树蛙蝌蚪对敌敌畏遗传毒性的敏感性[J].动物学报,2006,52(5):892-898.
作者姓名:耿宝荣  薛清清  连迎  姚丹
作者单位:福建师范大学生命科学学院,福州,350007
基金项目:国家科技攻关项目;福建省自然科学基金
摘    要:敌敌畏是我国农田中使用最普遍的有机磷杀虫剂,它通过干扰对神经传导起重要作用的胆碱酯酶的活性,起到接触和摄入毒杀昆虫的作用,也对当地水生动物及种群造成很大威胁。本文以广泛分布于我国南方农田区域的斑腿树蛙蝌蚪为研究对象,用碱性单细胞电泳方法比较了暴露于不同浓度(2.08,4.16,6.24,8.32,10.40mg/L)的敌敌畏溶液中三个发育阶段的遗传毒性的差异。结果表明:敌敌畏溶液暴露24h,对早期和中期阶段的蝌蚪造成极显著的DNA损伤(P<0.01),甚至在敌敌畏浓度低达2.08mg/L的溶液中也产生显著的损伤(P<0.05);统计分析表明,DNA长宽比率与敌敌畏剂量之间呈显著的线性关系,早期阶段和中期阶段的相关系数分别为0.950(P<0.01)和0.954(P<0.01)。对于后期阶段的蝌蚪,所有浓度的敌敌畏溶液都未导致明显的DNA损伤,作者认为进入变态阶段的蝌蚪对敌敌畏的敏感性明显下降。结果同时也表明,早期和中期阶段的斑腿树蛙蝌蝌是一种监测环境遗传毒性的合适指示生物。

关 键 词:斑腿树蛙蝌蚪  DNA损伤  遗传毒性  水体监测  慧星试验  发育时期  敌敌畏
收稿时间:2006-03-19
修稿时间:2006-03-192006-06-07

Comparison of sensitivity to Dichlorvos of Rhacophorus megacephalus tadpole at different developmental stages
GENG Bao-Rong,XUE Qing-Qing,LIAN Ying,YAO Dan.Comparison of sensitivity to Dichlorvos of Rhacophorus megacephalus tadpole at different developmental stages[J].Acta Zoologica Sinica,2006,52(5):892-898.
Authors:GENG Bao-Rong  XUE Qing-Qing  LIAN Ying  YAO Dan
Institution:College of Life Sciences, Fujian Normal University, Fuzhou LIAN Ying, YAO Dan 350007, China
Abstract:Dichlorvos is the most common organophosphate pesticide used in agricultural fields in China. It appears to be a contact and stomach poison in insects caused by interference with cholinesterase, a neurological enzyme which plays an important role in neurotransmission. The contamination of various bodies of water by run-off from fields treated with the chemical could have a negative impact on indigenous aquatic fauna. In this report, the sensitivities of treefrog Rhacophorus megacephalus tadpoles at early, middle and late developmental stages, respectively, to Dichlorvos at various concentrations(2.08 mg/L to 10.40 mg/L) were evaluated using alkaline single-cell gel electrophoresis assay(SCGE). The results showed that 24 h exposure to Dichlorvos induced significant DNA damage in tadpoles at early and middle stages(P<0.01) with significant increases(P<0.05) in the length-to-width ratios of the DNA patterns even at concentrations as low as 2.08 mg/L. Statistical analysis revealed linear correlations between the mean DNA length-to-width ratios and the doses of Dichlorvos at the first two developmental stages. The correlation coefficient values were 0.950(P<0.01) at early and 0.954(P<0.01) at middle stages. However, exposure to all treatment levels of Dichlorvos tested did not result in significant increases of DNA damage in tadpoles at late stage studied, suggesting that the sensitivity to Dichlorvos may diminish with the development of tadpoles to the metamorphic stage. These results indicate that the early and middle phases of R.megacephalus tadpoles may serve as a suitable tool for biomonitoring environmental genotoxicity using alkaline SCGE.
Keywords:Rhacophorus megacephalus-tadpoles  DNA damage  Genotoxicity  Aquatic monitoring  Alkaline SCGE  Developmental stage  Dichlorvos
本文献已被 CNKI 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号