| 三疣梭子蟹精子顶体反应前后胞内Ca~(2+)的变化 |
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| 引用本文： | 朱冬发,沈丽萍,沈建明,吴琼.三疣梭子蟹精子顶体反应前后胞内Ca~(2+)的变化[J].动物学报,2008,54(5). |
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| 作者姓名： | 朱冬发 沈丽萍 沈建明 吴琼 |
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| 作者单位： | 宁波大学应用海洋生物技术教育部重点实验室,浙江,宁波,315211 |
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| 基金项目： | 国家自然科学基金，浙江省科技计划，浙江省自然科学基金 |
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| 摘 要： | 应用激光扫描共聚焦显微镜(LSCM)和Fluo-3/AM荧染技术对三疣梭子蟹精子顶体反应前后的胞内Ca2 变化进行了观察和检测.结果显示,在精子顶体反应过程中,胞内Ca2 主要分布在细胞核、穿孔器和胞质膜残存处,胞内Ca2 浓度(Ca2 ]I)总体上呈现先上升后下降的趋势.顶体反应前精子的平均荧光强度为35.95±5.71;穿孔器前伸、顶体囊膜翻转阶段精子的平均荧光强度为66.80±7.35;顶体囊膜脱落、顶体丝形成阶段精子的平均荧光强度为3.87±2.82;上述各阶段间精子荧光强度有极显著差异(P<0.01).顶体反应穿孔器前伸、顶体囊膜翻转阶段的精子相比顶体反应前精子,Ca2 ]I显著提高;而在顶体囊膜脱落、顶体丝形成阶段,Ca2 ]I则急剧下降,只在顶体丝基部胞质膜残存处有微量Ca2 存在.初步探讨了三疣梭子蟹精子顶体反应前后胞内Ca2 变化的功能.
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| 关 键 词： | 三疣梭子蟹 精子 顶体反应 激光扫描共聚焦显微镜 胞内Ca2 |
Changes in intracellular Ca~(2+)of sperm fore-and-aft acrosome reaction in the swimming crab Portunus trituberculatus |
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| ZHU Dong-Fa,SHEN Li-Ping,SHEN Jian-Ming,WU Qiong.Changes in intracellular Ca~(2+)of sperm fore-and-aft acrosome reaction in the swimming crab Portunus trituberculatus[J].Acta Zoologica Sinica,2008,54(5). |
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| Authors: | ZHU Dong-Fa SHEN Li-Ping SHEN Jian-Ming WU Qiong |
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| Institution: | ZHU Dong-Fa,SHEN Li-Ping,SHEN Jian-Ming,WU QiongKey Laboratory of Applied Marine Biotechnology of Ministry of Education,Ningbo University,Ningbo 315211,China |
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| Abstract: | The acrosome reaction process of sperm in the swimming crab Portunus trituberculatus(Miers)consists of four consecutive stages:(1)swelling of the apical cap;(2)eversion of the acrosomal vesicle;(3)forward elongation of the perforatorium and reversal of the acrosomal vesicle membrane;and(4)shedding of the acrosomal vesicle membrane and formation of the acrosomal filament.In the present study,Laser Scanning Confocal Microscope(LSCM)was used to image spermatozoa stained with Fluo-3/AM at different stages of th... |
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| Keywords: | Portunus trituberculatus Sperm Acrosome reaction Laser Scanning Confocal Microscope Intracellular Ca~(2+) |
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