云南矮马耳缘组织成纤维细胞系的建立及其生物学特性

随着全球生物多样性及其遗传资源丧失程度的加剧 ,使得各国纷纷投入大量人力和财力 ,并以多种方式收集、保存和整理大自然赋予人类的宝贵财富 ,如美国典型培养物保藏中心 (AmericanTypeCultureCollection ,ATCC) ,欧洲动物细胞培养物保藏中心 (EuropeanCollectionofAnimalCellC

Establishment and characteristics of a Yunnan pony ear marginal fibroblast cell line

A Yunnan pony ear marginal tissue fibroblast cell line (NYPEM 2/2) was successfully established using the explant of the ear marginal tissue and then trypsinization the cells from the outgrowth. Observations on cell morphology and dynamic growth, analysis of karyotype and isoenzymes of lactate dehydrogenase and malate dehydrogenase were carried out. The expression of recombinant green fluorescence protein in the cells were also undertaken. The results showed that the population doubling time (PDT) of the cells was 24 h; the frequency of cell chromosome number to be 2n=64 was 92.9%; the banding patterns of the isozymes of the two enzymes had significant difference between the Yunnan pony ear marginal fibroblast cell line and the fibroblast cell lines of PEM 2/2, MSHEM 2/2 and BLCHE 2/2 derived from the Picdmont bovine ear, Mongolian ovine ear and Beijing local chicken embryo respectively. Tests for the contamination from bacteria, fungi or mycoplasma were negative; the transfection efficiency for the recombinant plasmid was 32.3%. This newly established cell line make the Yunnan pony breed, a national important genetic resource preserved at cell level, as well as will provide an effective experimental material for genetic studies on the Yunnan pony.