| 神经病靶标酯酶调控结构域结合蛋白筛选及其在COS-7细胞中的表达 |
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| 引用本文： | 陈瑞,杨琳,姚智,刘承云,李晓华,李琴,刘杰,常平安,李薇,伍一军.神经病靶标酯酶调控结构域结合蛋白筛选及其在COS-7细胞中的表达[J].动物学报,2005,51(5):840-844. |
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| 作者姓名： | 陈瑞 杨琳 姚智 刘承云 李晓华 李琴 刘杰 常平安 李薇 伍一军 |
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| 作者单位： | 1. 中国科学院动物研究所,农业虫害鼠害综合治理研究国家重点实验室,北京,100080;中国科学院研究生院,北京,100039
2. 中国科学院动物研究所,农业虫害鼠害综合治理研究国家重点实验室,北京,100080 |
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| 基金项目： | 国家自然科学基金;中国科学院知识创新工程项目;国家高技术研究发展计划(863计划) |
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| 摘 要： | 本研究采用酵母双杂交系统探寻与神经病靶标酯酶（NTE）调控结构域相互作用的蛋白因子，揭示与NTE信号转导相关的可能机制。通过构建含有NTE调控结构域的诱饵蛋白载体筛选胎脑文库，并将筛选得到的阳性克隆在酵母中进行了验证，随后在哺乳动物细胞中表达了该蛋白。生物信息学分析显示：该阳性克隆为前列腺素受体结合蛋白54（ARA54），具有泛素连接酶活性，提示细胞可能存在依赖于细胞周期的NTE活性调节机制，为阐明NTE生理功能创造了条件动物学报51（5）：840—844，2005]。
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| 关 键 词： | 神经病靶标酯酶 酵母双杂交系统 泛素 ARA54 |
| 收稿时间： | 2005-03-16 |
| 修稿时间： | 2005-03-162005-06-06 |
Hunting for novel proteins in neuropathy target esterase regulatory domain with yeast two-hybrid system |
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| CHEN Rui,YANG Lin,YAO Zhi,LIU Cheng-Yun,LI Xiao-Hua,LI Qin,LIU Jie,CHANG Ping-An,LI Wei,WU Yi-Jun.Hunting for novel proteins in neuropathy target esterase regulatory domain with yeast two-hybrid system[J].Acta Zoologica Sinica,2005,51(5):840-844. |
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| Authors: | CHEN Rui YANG Lin YAO Zhi LIU Cheng-Yun LI Xiao-Hua LI Qin LIU Jie CHANG Ping-An LI Wei WU Yi-Jun |
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| Institution: | 1. State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, The Chinese Academy of Sciences, Beijing 100080, China 2.Graduate School of the Chinese Academy of Sciences, Beijing 100039, China |
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| Abstract: | To explore protein factors that may interact with regulatory domain of neuropathy target esterase (NTE) in signal transduction pathways,the bait plasmid of NTE-regulatory domain cDNA was constructed and screened in human fetal brain cDNA library by the method of yeast two-hybrid.The candidate positive clones were retested in yeast,and its expression was verified in mammalian cells.The results of bioinformatics showed that androgen receptor-associated protein 54 (ARA54),a ubiquitin protein ligase,may be an essential controller of the NTE in the process of cell cycle.This result will put great opportunity for understanding the physiological mechanism of the NTE. |
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| Keywords: | Neuropathy target esterase Yeast two-hybrid system Ubiquitin ARA54 |
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