中国大鲵消化系统13种器官的蛋白水解酶种类和活性分析

蛋白水解对生命活动是必不可少的(Vassali et al., 1994),蛋白质的酶解修饰(Xu et al.,1999)、细胞的迁移、组织再生与修复、消化系统对食物中蛋白质的消化等均与蛋白水解酶有关(Baimbridge et al.,1992),许多病理过程也与蛋白水解酶功能失调有关(Teichert et al., 1989; Monard, 1988).因此开展大鲵消化系统各器官的蛋白水解酶种类和性质的研究,对了解大鲵消化系统各器官的功能、演化及大鲵的营养需求、食性、消化生理等是必要的.本文对大鲵消化系统各器官的蛋白水解酶特征进行了初步分析,现将结果报道如下.

Analysis of the proteases activity in 13 organs of the doll fish (Andrias davidianus) digestive system

Protease activity in 13 doll fish, Andrias davidianus, digestive organs: tongue, esophagus, cardia, stomach, pylorus, duodenum, jejunum, ileum, rectum, cloacal chamber, liver, pancreas, gall bladder were analysed with gelatine polyacrylamide gel-electrophoresis (G-PAGE). Compared to rats and rhesus monkeys (Macaca mullatta tcheliensis), protease activity in the Andrias davidianus digestive system was more varied and stronger. The activity of acid proteases>that of the neutral proteases>that of the alkaline proteases. The kinds and activities of neutral and alkaline proteases in the Andrias davidianus digestive system wereare very different, while the kinds of acid proteases in them were very similar, but their activities are different. Analysing the activities of proteases in these organs shows that protease activity in the duodenum>that in rectum>that in stomach>that in pancreas>that in liver. Experimental results suggest that the function of the rectum and liver in Andrias davidianus may be as important as that of the duodenum and pancreas in food degradation .