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体外培养条件下促性腺激素对昆明白小鼠卵母细胞成熟及卵丘扩展的影响
引用本文:陈勇,夏国良,苏友强,王海滨,赫晨.体外培养条件下促性腺激素对昆明白小鼠卵母细胞成熟及卵丘扩展的影响[J].动物学报,2001,47(2):203-208.
作者姓名:陈勇  夏国良  苏友强  王海滨  赫晨
作者单位:中国农业大学生物学院,
基金项目:国家自然科学基金,教育部留学回国人员科研启动基金,39770396,,,
摘    要:研究促卵泡激素(FSH),人绒毛膜促性腺激素(hCG)对昆明白小鼠卵母细胞成熟和卵丘扩展的影响,以及体外培养时卵丘扩展与卵母细胞成熟之间的关系,FSH可以明显促进次黄嘌吟(HX)抑制条件下的卵丘-卵母细胞复合体CEO卵母细胞成熟及卵丘扩展,其最佳作用剂量为100IU/L,且FSH作用30分钟即可以使CEO获得恢复减数分裂的信息,在HX存在的条件下,FSH处理后10hr,CEO卵丘明显扩展,而生发泡破裂(GVBD)则在16-20hr明显增加,所有卵丘未扩展的CEO中卵母细胞均未发生GVBD,低剂量hCG单独或与FSH共同存在,对CEO卵母细胞成熟及卵丘扩展均无明显影响;高剂量hCG可以部分抑制FSH对卵母细胞成熟的促进作用,结果表明:FSH明显促进CEO卵母细胞成熟及卵丘扩展,而hCG却不具有此作用,体外培养条件下(含次黄嘌呤),卵丘扩展是卵母细胞成熟的前提条件,但卵母细胞成熟并不需要卵丘完全扩展。

关 键 词:昆明白小鼠  卵丘扩展  卵母细胞成熟  促性腺激素

EFFECT OF GONADOTROPINSON IN VITRO MATURATION OF OOCYTE AND CUMULUS EXPANSION IN KUNMING WHITE MOUSE
CHEN Yong,XIA Guo-liang,SU You-Qiang,WANG Hai-Bin,HE Chen.EFFECT OF GONADOTROPINSON IN VITRO MATURATION OF OOCYTE AND CUMULUS EXPANSION IN KUNMING WHITE MOUSE[J].Acta Zoologica Sinica,2001,47(2):203-208.
Authors:CHEN Yong  XIA Guo-liang  SU You-Qiang  WANG Hai-Bin  HE Chen
Abstract:The present experiments were conducted to evaluate the effect of gonadotro pins on Kunming white mouse oocyte maturation and the relationship between cumul us cell expansion and oocyte maturation in vitro. The cumulus cell enclosed oo cytes (CEO) were collected from gonadotropins stimulated Kunming white mouse ov ari es and were cultured for 24hr in 4 mmol/L hypoxthantine (HX) supplemented me dium (HX medium) and treated with different agents in the following designs: (1) CE O were treated with FSH (10~500 IU/L) to find the optimal dosage of FSH on the oocyte maturation; (2) CEO were treated with 100 IU/L FSH for the different time (0, 10, 30, 60 and 120 min) and then transferred to a new HX medium for the fur t her culture to figure out the time effect of FSH on oocyte maturation and cumulu s cell expansion; (3) CEO were treated with different dose of hCG (0~400 IU/L) or hCG plus FSH to evaluate the effect of hCG on oocyte maturation and cumulus cell expansion. The results revealed that FSH stimulated oocyte resumption of me iosis and cumulus expansion of cumulus cell enclosed oocyte(CEO)in the presen ce of 4 mol/L hypoxathine(HX), with maximal effect occurring at a dosage of 100 IU /L. The oocyte meiotic resumption could be induced by 30 min FSH presentation. By 10 hr after FSH treatment, cumulus cells had already apparently expanded, where as the significant increase of GVBD did not appear until 16~20 hr in culture. Ne arly all the oocytes with cumulus cell expansion underwent the meiotic resumptio n. But those CEO whose cumulus cell did not expanded didn't undergo GVBD. Low do ses of hCG failed to affect the oocyte maturation and cumulus expansion of CEO, however, high doses of hCG could partially inhibit the oocyte maturation stimula ted by FSH. Our results indicate that, in the presence of HX, FSH stimulated sig nificantly cumulus expansion and oocyte maturation of CEO. hCG had no stimulation effect on the oocyte meiotic resumption. The cumulus cell expansion occurred 6 to 8hr before the oocyte resumption of meiosis. We conclude that oocyte meioti c resumption stimulated by FSH in the presence of HX in vitro is initiated by cum ulus expansion, whereas cumulus mass complete expansion is not needed for the ma turation of oocyte.
Keywords:Kunming white mouse  Oocyte resumption of meiosis  Cumulus expansion    Gonadotropins
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