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维生素C多聚磷酸酯对小鼠肝脏脂质过氧化物和抗氧化物酶的影响
引用本文:周显青,李胜利,王晓辉,乔欣.维生素C多聚磷酸酯对小鼠肝脏脂质过氧化物和抗氧化物酶的影响[J].动物学报,2004,50(3):370-374.
作者姓名:周显青  李胜利  王晓辉  乔欣
作者单位:首都医科大学实验动物科学部,北京,100054
基金项目:北京市教育委员会课题资助 ( 2 0 0 2KJ0 85 )~~
摘    要:为研究维生素C多聚磷酸酯对小鼠肝脏脂质过氧化物和抗氧化物酶的影响 ,我们设置了 4个实验组 ,采用 2 4只小鼠 ,饵料中 35 %维生素C多聚磷酸酯的添加量依次为 0、 5 0 0、 2 5 0 0和 5 0 0 0mg/kg ,喂食 4周后取其肝脏 ,用硫代巴比妥酸分光光度测脂质过氧化物的含量 ,用亚硝酸盐形成法测定超氧化物歧化酶的活性 ,用分光光度法测过氧化氢酶和谷胱甘肽过氧化物酶的活性。结果表明 ,维生素C多聚磷酸酯对小鼠肝脏脂质过氧化物没有明显影响 ,但随着维生素C多聚磷酸酯添加量的增加 ,脂质过氧化物有减少的趋势。维生素C多聚磷酸酯添加量为 2 5 0 0和 5 0 0 0mg/kg的两组 ,其超氧化物歧化酶的活性明显高于对照组和维生素C多聚磷酸酯添加量为 5 0 0mg/kg组 ;过氧化氢酶的活性明显高于对照组。维生素C多聚磷酸酯添加量为5 0 0 0mg/kg组 ,其谷胱甘肽过氧化物酶的活性明显高于其它三组。表明高剂量的维生素C多聚磷酸酯能促进小鼠抗氧化物酶的活性 ,但促进不同抗氧化物酶活性所需的维生素C多聚磷酸酯的量不同

关 键 词:小鼠  维生素C多聚磷酸酯  脂质过氧化物  超氧化物歧化酶  过氧化氢酶  谷胱甘肽过氧化物酶

Effects of vitamin C polyphosphate on liver lipid peroxides and antioxidase activity in mice
ZHOU Xian Qing ,LI Sheng Li,WANG Xiao Hui,QIAO Xin.Effects of vitamin C polyphosphate on liver lipid peroxides and antioxidase activity in mice[J].Acta Zoologica Sinica,2004,50(3):370-374.
Authors:ZHOU Xian Qing  LI Sheng Li  WANG Xiao Hui  QIAO Xin
Institution:ZHOU Xian Qing **,LI Sheng Li,WANG Xiao Hui,QIAO Xin Department of Laboratory Animal Sciences,Capital Medical University,Beijing 100054,China
Abstract:The study was conducted to investigate the effects of vitamin C polyphosphate on liver lipid peroxides (LPO) and antioxidase activity in mice. Twenty four mice were fed with 35% vitamin C polyphosphate supplementation at the dosages of 0, 500, 2 500 , 5 000 mg/kg diets, respectively for 4 weeks. Livers were collected to analyze the content of LPO, and the activities of the superoxide dismutases (SOD), catalase (CAT) and glutathione peroxidase (GSH Px) . The LPO and SOD were determined with thiobarbituric acid reaction and nitrite form method, respectively; and the CAT and GSH Px with colorimetry method. The results showed that vitamin C polyphosphate had no significant effects on liver LPO levels in mice, although there was a decreasing tendency with an increase of vitamin C polyphosphate levels. The activities of SOD in the two groups supplemented vitamin C polyphosphate at 2 500 and 5 000 mg/kg diets were significantly higher than those of both control groups and the group supplemented vitamin C polyphosphate at 500 mg/kg. The activities of CAT in the two groups supplemented vitamin C polyphosphate at 2 500 and 5 000 mg/kg diets were higher than that of control group. The activity of GSH Px in the groups supplemented vitamin C polyphosphate at 5 000 mg/kg diets was notably higher than those of other three groups. These results suggest that vitamin C polyphosphate can improve the antioxidase activity, while the requirement of vitamin C polyphosphate for improving different antioxidase activity varies .
Keywords:Mice  Vitamin C polyphosphate  Lipid peroxide  Superoxide distutase  Catalase  Glutathione peroxidase
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