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Genetic diversity and biogeography of rhizobia associated with Caragana species in three ecological regions of China
Authors:Yang Li Lu  Wen Feng Chen  En Tao Wang  Su Hua Guan  Xue Rui Yan  Wen Xin Chen
Institution:1. The State Key Laboratory for Agrobiotechnology/Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing 100193, China;2. Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México D. F. 11340, Mexico;3. College of Plant Protection, Shenyang Agricultural University, Shenyang 110161, China
Abstract:Twenty-two genospecies belonging mainly to Mesorhizobium, and occasionally to Rhizobium and Bradyrhizobium, were defined among the 174 rhizobia strains isolated from Caragana species. Highly similar nodC genes were found in the sole Bradyrhizobium strain and among all the detected Mesorhizobium strains. A clear correlation between rhizobial genospecies and the eco-regions where they were isolated was found using homogeneity analysis. All these results demonstrated that Caragana species had stringently selected the rhizobia symbiotic genotype, but not the genomic background; lateral transfer of symbiotic genes from Mesorhizobium to Bradyrhizobium and among the Mesorhizobium species has happened in the Caragana rhizobia; and biogeography of Caragana rhizobia exists. Furthermore, a combined cluster analysis, based upon the patterns obtained from amplified 16S rRNA gene and 16S–23S intergenic spacer restriction analyses, BOX PCR and SDS-PAGE of proteins, was reported to be an efficient method to define the genospecies.
Keywords:Caragana  Mesorhizobium  Diversity  Eco-region  Combined cluster analysis
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