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Govania unica gen. nov., sp. nov., a rare biosphere bacterium that represents a novel family in the class Alphaproteobacteria
Institution:1. Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, K. L. Ledeganckstraat 35, 9000 Ghent, Belgium;2. LIPME, Université de Toulouse, INRAE, CNRS, Castanet-Tolosan, France;1. Centre for Marine Science and Innovation, School of Biological, Earth and Environmental Sciences, University of New South Wales, Sydney, New South Wales, Australia;2. Australian Institute of Marine Science, Townsville, Queensland, Australia;3. Cawthron Institute, Nelson, New Zealand;4. Unit of Microbiome Science and Biotechnology, Department of Pharmacy and Biotechnology, University of Bologna, via Belmeloro 6, 40126 Bologna, Italy;5. University of Haifa, Haifa, Israel;6. Australian Centre for Ecogenomics, School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD, Australia;7. Australian Antarctic Division, Kingston, Tasmania, Australia;1. The National Institute of Horticultural Research, Department of Phytopathology, ul. Konstytucji 3 Maja 1/3, 96-100 Skierniewice, Poland;2. Julius Kühn Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Plant Protection in Horticulture and Forests, Messeweg 11-12, 38104 Braunschweig, Germany;1. College of Oceanology and Food Science, Quanzhou Normal University, Quanzhou, China;2. Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, Quanzhou, China;3. Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, China;1. Marine Microbiology Group, Department of Animal and Microbial Biodiversity, Mediterranean Institute for Advanced Studies (IMEDEA, CSIC-UIB), Esporles, Spain;2. Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Bremen, Germany;3. Ocean Science & Engineering, School of Biological Sciences, Georgia Institute of Technology, Atlanta, GA, USA;4. School of Civil & Environmental Engineering, Georgia Institute of Technology, Atlanta, GA, USA;5. Research Unit Analytical BioGeoChemistry, Helmholtz Munich, 85764 Neuherberg, Germany;6. Chair of Analytical Food Chemistry, Technical University Munich, Maximus-von-Imhof-Forum 2, 85354 Freising, Germany;7. Department of Physiology, Genetics and Microbiology, University of Alicante, 03690, San Vicent del Raspeig, Alicante, Spain;8. Laboratorio de Ecología Acuática, Instituto Tecnológico Chascomús (INTECH)-CONICET-UNSAM, Escuela de Bio y Nanotecnologías -UNSAM, Buenos Aires, Argentina;9. School of Life Sciences, University of Nevada, Las Vegas, NV 89154-4004, USA;10. Doctoral School of Integrative Biology, Faculty of Biology and Geology, Babe?-Bolyai University, Cluj-Napoca, Romania;11. Department of Biochemistry, Genetics and Microbiology, and Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, South Africa;12. Great Salt Lake Institute, Westminster College, Salt Lake City, UT, 84105, USA;13. Department of Taxonomy and Ecology, Faculty of Biology and Geology, Babe?-Bolyai University, Cluj?Napoca, Romania;14. Department of Molecular Biology and Biotechnology, Faculty of Biology and Geology, Babe?-Bolyai University, Cluj?Napoca, Romania;15. Emil G. Racovi?? Institute, Babe?-Bolyai University, Cluj?Napoca, Romania;p. School of Biological Sciences, University of Canterbury, Christchurch, New Zealand;q. Institute of Applied Microbiology (IFZ), Justus Liebig Universität Giessen, Giessen, Germany;1. Centre for Marine Science and Innovation, School of Biological, Earth and Environmental Sciences, University of New South Wales, Sydney, New South Wales, Australia;2. Australian Institute of Marine Science, Townsville, Queensland, Australia;3. Australian Antarctic Division, Hobart, Tasmania, Australia;1. Marine Microbiology Group, Department of Animal and Microbial Biodiversity, Mediterranean Institute for Advanced Studies (CSIC-UIB), 070190 Esporles, Spain;2. School of Civil and Environmental Engineering, and School of Biological Sciences, Georgia Institute of Technology, Atlanta, GA, USA;3. Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Celsiusstrasse 1, D-28359 Bremen, Germany
Abstract:Strain LMG 31809 T was isolated from a top soil sample of a temperate, mixed deciduous forest in Belgium. Comparison of its 16S rRNA gene sequence with that of type strains of bacteria with validly published names positioned it in the class Alphaproteobacteria and highlighted a major evolutionary divergence from its near neighbor species which represented species of the orders Emcibacterales and Sphingomonadales. 16S rRNA amplicon sequencing of the same soil sample revealed a highly diverse community in which Acidobacteria and Alphaproteobacteria predominated, but failed to yield amplicon sequence variants highly similar to that of strain LMG 31809 T. There were no metagenome assembled genomes that corresponded to the same species and a comprehensive analysis of public 16S rRNA amplicon sequencing data sets demonstrated that strain LMG 31809 T represents a rare biosphere bacterium that occurs at very low abundances in multiple soil and water-related ecosystems. The genome analysis suggested that this strain is a strictly aerobic heterotroph that is asaccharolytic and uses organic acids and possibly aromatic compounds as growth substrates. We propose to classify LMG 31809 T as a novel species within a novel genus, Govania unica gen. nov., sp. nov, within the novel family Govaniaceae of the class Alphaproteobacteria. Its type strain is LMG 31809 T (=CECT 30155 T). The whole-genome sequence of strain LMG 31809 T has a size of 3.21 Mbp. The G + C content is 58.99 mol%. The 16S rRNA gene and whole-genome sequences of strain LMG 31809 T are publicly available under accession numbers OQ161091 and JANWOI000000000, respectively.
Keywords:Novel species  Taxonomy  Comparative genomics  Rare biosphere  ASVs"}  {"#name":"keyword"  "$":{"id":"k0035"}  "$$":[{"#name":"text"  "_":"amplicon sequence variants  MALDI-TOF MS"}  {"#name":"keyword"  "$":{"id":"k0045"}  "$$":[{"#name":"text"  "_":"matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry  TEM"}  {"#name":"keyword"  "$":{"id":"k0055"}  "$$":[{"#name":"text"  "_":"transmission electron microscopy
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