Microbial communities involved in biogas production from wheat straw as the sole substrate within a two-phase solid-state anaerobic digestion |
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| Institution: | 1. Leibniz Institute for Agricultural Engineering Potsdam-Bornim, Max-Eyth-Allee 100, 14469 Potsdam, Germany;2. Technische Universität Berlin, Faculty of Process Sciences, Straße des 17. Juni 135, 10623 Berlin, Germany;1. School of Civil Engineering, Beijing Jiaotong University, Beijing 100044, China;2. State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, China;3. College of Science, Hunan Agricultural University, Changsha 410128, China;1. Advanced Water Management Centre, The University of Queensland, Brisbane, QLD, 4072, Australia;2. Department of Microbiology, Quaid-i-Azam University, Islamabad, Pakistan;3. U.S. Pakistan Center for Advanced Studies in Energy, National University of Sciences and Technology, Islamabad, Pakistan;1. INRA, UR 050 Laboratoire de Biotechnologie de l’Environnement, Avenue des étangs, F-11100 Narbonne, France;2. LISBP – INSA de Toulouse, INSA/CNRS 5504 – UMR INSA/INRA 792, 135 Avenue de Rangueil, 31077 Toulouse CEDEX 04, France;3. INRA, UMR 1208 Ingénierie des Agropolymères et Technologies Emergentes INRA-CIRAD-Supagro-UMII, 2 Place Viala, F-34060 Montpellier, France;1. Shandong Industrial Engineering Laboratory of Biogas Production & Utilization, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong Province 266101, PR China;2. University of Chinese Academy of Sciences, Beijing 100049, PR China;1. State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin 150090, China;2. School of Chemical and Environmental Engineering, Liaoning University of Technology, Liaoning 121001, China |
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| Abstract: | Microbial communities involved in biogas production from wheat straw as the sole substrate were investigated. Anaerobic digestion was carried out within an up-flow anaerobic solid-state (UASS) reactor connected to an anaerobic filter (AF) by liquor recirculation. Two lab-scale reactor systems were operated simultaneously at 37 °C and 55 °C. The UASS reactors were fed at a fixed organic loading rate of 2.5 g L?1 d?1, based on volatile solids. Molecular genetic analyses of the bacterial and archaeal communities within the UASS reactors (digestate and effluent liquor) and the AFs (biofilm carrier and effluent liquor) were conducted under steady-state conditions. The thermophilic UASS reactor had a considerably higher biogas and methane yield in comparison to the mesophilic UASS, while the mesophilic AF was slightly more productive than the thermophilic AF. When the thermophilic and mesophilic community structures were compared, the thermophilic system was characterized by a higher Firmicutes to Bacteroidetes ratio, as revealed by 16S rRNA gene (rrs) sequence analysis. The composition of the archaeal communities was phase-separated under thermophilic conditions, but rather stage-specific under mesophilic conditions. Family- and order-specific real-time PCR of methanogenic Archaea supported the taxonomic distribution obtained by rrs sequence analysis. The higher anaerobic digestion efficiency of the thermophilic compared to the mesophilic UASS reactor was accompanied by a high abundance of Firmicutes and Methanosarcina sp. in the thermophilic UASS biofilm. |
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| Keywords: | Anaerobic digestion Biogas Biomethanization Microbial community Wheat straw |
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