Normal human endometrium in cell culture |
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Authors: | D Kirk R J B King Judy Heyes Linda Peachey P J Hirsch R W T Taylor |
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Institution: | (1) Department of Hormone Biochemistry, Lincoln's Inn Fields, WC2A 3PX London;(2) Department of Pathology, Imperial Cancer Research Fund, Lincoln's Inn Fields, WC2A 3PX London;(3) Hope Hospital, Eccles Old Road, M6 8HD Salford;(4) Department of Obstetrics and Gynaecology, St. Thomas' Hospital, SE1 7EH London |
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Abstract: | Summary Separation of human endometrium into its epithelial and stromal components has been achieved through collagenase digestion
and has permitted a study of these two cell populations under specific experimental culture conditions. The stromal cell populations
showed a progesterone response, were easily handled in culture, and displayed a limited in vitro life span typical of human
diploid fibroblasts. In contrast, epithelium only survived in shortterm primary culture and showed no clear hormone response.
High-density epithelial cultures remained viable for longer periods in culture. Comparisons between resurfacing endometrial
epithelial cells in vivo and epithelial cells migrating from explants in vitro suggested that this initial epithelial migration
in vitro was the counterpart of the repair response in vivo.
We are much in debt to Dr. R. C. Hallowes (Department of Pathology, Imperial Cancer Research Fund) for his guidance and encouragement
throughout the course of this work. We also gratefully acknowledge Dr. P. N. Riddle (Time-Lapse Cinematography Unit, Imperial
Cancer Research Fund) for carrying out the time-lapse cinematography; Mrs. Lyn Rolph (Stereoscan Unit, Bedford College, University
of London) for assisting with the SEM; and Mr. G. D. Leach for his competent help with the photography. |
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Keywords: | cell culture human endometrium epithelium stroma |
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