Plant regeneration in <Emphasis Type="Italic">Arachis stenosperma</Emphasis> Krapov. and W. C. Gregory from roots and calluses derived from leaflets of <Emphasis Type="Italic">in vitro</Emphasis> plants |
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| Authors: | Georgia Pacheco Rachel Fatima Gagliardi Leonardo Alves Carneiro José Francisco Montenegro Valls Elisabeth Mansur |
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| Institution: | 1.Laboratório de Micropropaga??o e Transforma??o de Plantas,DBCG-Universidade do Estado do Rio de Janeiro,Rio de Janeiro,Brazil;2.Embrapa Recursos Genéticos e Biotecnologia,Brasília,Brazil;3.Laboratório de Micropropaga??o e Transforma??o de Plantas,Universidade do Estado do Rio de Janeiro, IBRAG, DBCG,Rio de Janeiro,Brazil |
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| Abstract: | Seed explants of A. stenosperma were cultured on MS medium supplemented with 6-benzylaminopurine with the aim of rescuing nonviable accessions stored in
seed bank conditions. The regeneration potential of leaf explants from in vitro plants derived from embryonic axes was studied by using whole leaflets and leaflet segments. Explants were cultured on Murashige
and Skoog (MS) medium supplemented with different concentrations of 6-benzylaminopurine and naphthalene acetic acid. Indirect
organogenesis was observed in response to 6-benzylaminopurine, either alone or in association with naphthalene acetic acid,
in both explant types. Media supplemented with naphthalene acetic acid as the sole growth regulator induced rhizogenesis in
whole leaflets and leaflet segments, with subsequent shoot production directly from the roots. |
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| Keywords: | Groundnut Micropropagation Organogenesis Rhizogenesis |
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