In vitro propagation of the medicinal plant Plumbago zeylanica L. Through nodal explants |
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Authors: | V Selvakumar P R Anbudurai T Balakumar |
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Institution: | (1) Centre for Plant Biochemistry and Molecular Biology, Department of Botany, The American College, 625 002 Madurai, India |
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Abstract: | Summary A protocol for rapid in vitro propagation using nodal explants obtained from 2-yr-old, field-grown medicinal plants of Plumbago zeylanica L. belonging to the family Plumbaginaceae is described. High frequency bud break and fast development of shoots were induced
on Murashige and Skoog's basal medium supplemented with 27.2 μM adenine sulfate +2.46 μM indole-3-butyric acid (IBA). Induction of rooting was achieved by transferring the shoots to the same basal medium containing
4.92 μM IBA. Using our protocol from one twig of P. zeylanica (eight responsive nodes per explant shoot) within a period of 5 mo., eight plantlets could be raised. After a hardening period
of 4 wk, there was a 90% transplantation success in the field compared to the 60–65% survival of plantlets recorded in the
experiments of previous workers. The plantlets derived through in vitro propagation mimic the growth and morphological characteristics of the donor plants. |
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Keywords: | Plumbago zeylanica axillary bud development rooting medicinal plant |
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