Recovery and evaluation of soybean plants transgenic for aBacillus thuringiensis var.Kurstaki insecticidal gene |
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Authors: | W A Parrott J N All M J Adang M A Bailey H R Boerma C N Stewart Jr |
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Institution: | (1) Department of Crop and Soil Sciences, The University of Georgia, 30602 Athens, Georgia;(2) Department of Entomology, The University of Georgia, 30602 Athens, Georgia |
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Abstract: | Summary Lepidopteran insects are major defoliating pests of soybean in the southeastern United States. Soybean plants transgenic for
a nativecryIA(b) gene fromBacillus thuringiensis var.kurstaki HD-1 were obtained. Embryogenic cultures were induced by plating cotyledons on a Murashige and Skoog-based medium supplemented
with 40 mg/liter of 2,4-dichlorophenoxyacetic acid (2,4-D). The embryogenic cultures were maintained in liquid medium containing
5 mg/liter 2,4-D. These cultures were subjected to microprojectile bombardment, followed by selection on 50 mg/liter hygromycin.
Resistant embryogenic cell lines were transferred to growth regulator-free medium to permit recovery of mature somatic embryos.
After a desiccation period, the somatic embryos were returned to growth regulator-free medium for conversion into plants.
Southern hybridization analysis verified transformation. Feeding assays of T1 plants from one cell line deterred feeding, development, and survival of velvetbean caterpillar at a level comparable to
that of GatIR81-296, a soybean breeding line with a high level of insect resistance. Reduced feeding on T1 plants correlated with the presence of the transgene. |
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Keywords: | Bacillus thuringiensis insect resistance microprojectile bombardment somatic embryogenesis soybean transformation |
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