Comparison of coulter volumes with radiometrically determined intracellular water volumes for cultured cells |
| |
Authors: | Neal S Burres Carol E Cass |
| |
Institution: | (1) McEachern Laboratory and Department of Biochemistry, University of Alberta, T6G 2H7 Edmonton, Alberta, Zanada;(2) Present address: Harbor Branch Oceanographic Institution, 5600 Old Dixie Highway, 33450 Ft. Pierce, Florida |
| |
Abstract: | Summary During methotrexate-induced differentiation of cultured human choriocarcinoma (BeWo) cells, proliferation is inhibited, morphologic
and biochemical changes occur, and giant, often multinucleated, cells form. We have used the increase in cell volume as a
marker of the mature syncytiotrophoblastlike phenotype. Uninduced and differentiated BeWo cells are not spherical, and theoretical
considerations suggested that deviations in shape could result in significant errors in Coulter volume. To determine if the
values obtained by electrical pulse sizing reflected the actual mass of BeWo cells, we have evaluated the relationship between
Coulter volumes and intracellular water volumes obtained using a shape-independent estimate for eight cell types. A close
correlation (r
2=0.97) was found, indicating that cell volume changes in populations of irregularly shaped cells can be accurately measured
using a Coulter instrument.
Supported by an operating grant from the National Cancer Institute of Canada. N.S.B. was a recipient of a studentship award
from the Alberta Heritage Foundation for Medical Research. C.E.C. is a Senior Research Scientist of the National Cancer Institute
of Canada. The McEachern Laboratory is a research facility of the Faculty of Medicine, University of Alberta, and the Cross
Cancer Institute, Edmonton, Alberta. |
| |
Keywords: | BeWo cells cell volume Coulter principle |
本文献已被 SpringerLink 等数据库收录! |
|