Embryoid formation in alfalfa cell suspension cultures from different plants

Summary Cell suspension cultures were initiated from shoot tips of nine plants of alfalfa. Our results indicated that considerably different nutritional conditions were required to induce embryogenesis in the cell cultures derived from these plants. By proper adjustment of the hormone level and mineral salt concentration it was possible to induce embryogenesis in all of the nine cultures tested. The embryoids from seven of the nine cultures were able to develop into plants.