Plant regeneration from shoot tips and callus of papaya |
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Authors: | Shi-Tao Yie S I Liaw |
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Institution: | (1) Research Institute of Botany, National Chung-Hsing University, Taichung, Taiwan, Republic of China |
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Abstract: | Summary Two methods of in vitro culture were employed to regenerate papaya plants. One involved regeneration of plants from callus
and the other, production of multiple plants from single shoot-tip explants. Callus was induced from stem sections of papaya
seedlings in a medium containing 1 mg per 1 NAA and 0.1 mg per 1 kinetin. The callus regenerated shoots and/or embryoids when
transferred to a medium of lower auxin, 0 to 0.05 mg per 1 IAA, and higher cytokinin, 1 to 2 mg per 1 kinetin Multiple shoots
were produced when the excised shoot-tip explants were cultured in a medium supplemented with 0.05 mg per 1 IAA and either
5 mg per 1 kinetin or 0.5 to 1.0 mg per 1 benzyladenine. Root formation of the shoots or embryoids that derived from callus
or shoot tips occurred in a medium containing 5 mg per 1 IAA and in a light intensity of 3000 to 4000 Ix. The rooted plants
could be established in soil and under standard greenhouse conditions after they had been acclimated by initially growing
them in moist vermiculite contained in polyethylene-covered pots.
This research was supported by the National Science Council, Republic of China. |
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Keywords: | papaya callus plant regeneration shoot tips multiple shoots |
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