In vitro culture of tissue from the tunicateStyela clava |
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Authors: | David A Raftos Dan L Stillman Edwin L Cooper |
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Institution: | (1) Department of Anatomy and Cell Biology, UCLA School of Medicine, University of California at Los Angeles, 10833 Leconte Avenue, 90024 Los Angeles, California |
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Abstract: | Summary Pharyngeal explants and circulatory hemocytes from the tunicateStyela clava were cultured in a medium containing tunicate plasma, artificial seawater, RPMI 1640, and antibiotics. Pharnngeal tissue
remained viable and proliferated for up to 72 d in vitro. Proliferative activity maintained the pool of hemocytes within explants
and facilitated the migration of pharyngeal hemocytes from explants into culture supernatants. The diversity of morphologically
distinct cell types within the hemocyte pool of pharyngeal cultures indicated that cell division was followed by regulated
differentiation. In contrast to pharyngeal cultures, suspensions of circulatory hemocytes did not survive for prolonged periods
in vitro. Proliferative activity could not be detected in circulatory hemocyte cultures. These results are discussed in terms
of the differentiation state of hemocytes and the efficacy of culture conditions.
This study was supported by the National Science Foundation, Washington, DC (grant DCB 85 19848) and by BRSG funds from UCLA
Schools of Medicine and Dentistry. Flow cytometric facilities were sponsored in part by a Johnson Cancer Center Core Grant
(CA 16042). David A. Raftos is a Fulbright Postdoctoral Fellow and recipient of a Frederik B. Bang Scholarship in Marine Invertebrate
Immunology administered by the American Association of Immunologist. Dan L. Stillman was supported by an REU supplement from
the National Science Foundation. |
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Keywords: | primary tissue culture pharynx cell proliferation tunicate explants hemocytes |
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