Modified single node culture method—a new micropropagation method for chickpea |
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Authors: | K S Barna A K Wakhlu |
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Institution: | (1) Present address: Division of Biochemistry, Indian Agricultural Research Institute, 110012 New Delhi, India;(2) Department of Biosciences, University of Jammu, 180004 Jammu, India |
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Abstract: | Chickpea was micropropagated by axillary shoot proliferation (ASP) and modified single node culture (MSNC) methods. Maximum
propagule proliferation occurred on Murashige and Skoog (MS) medium enriched with 1–10 μM N6-benzyladenine and 0.01 μM α-naphthaleneacetic acid. The propagules were rooted on MS medium containing 1 μM 3-indolebutyric acid and B5 vitamins. Regenerated plants were fertile and phenotypically similar to control plants grown from seed. The MSNC method was
four times more efficient than the ASP method in terms of the number of plants produced per explant. |
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Keywords: | axillary shoot proliferation comparison efficiency modified single node culture propagules |
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