<Emphasis Type="Italic">In vitro</Emphasis> propagation of <Emphasis Type="Italic">Pluchea lanceolata</Emphasis>, a medicinal plant,and effect of heavy metals and different aminopurines on quercetin content |
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Authors: | Sanjeev?Kumar Alka?Narula M?P?Sharma Email author" target="_blank">P?S?SrivastavaEmail author |
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Institution: | (1) Amity Institute of Biotechnology, Amity IT University Campus, Sector 125, 201 301 Noida, Uttar Pradesh, India;(2) Centre for Biotechnology, Hamdard University, 110 062 New Delhi, India;(3) Department of Botany, Faculty of Science, Hamdard University, 110 062 New Delhi, India |
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Abstract: | Summary An effcient regeneration protocol has been developed from leaf explants of Pluchea lanceolata, a medicinally important plant of the family Asteracea. Nodular callus was initiated from young leaf segments cultured on Wood and Braun medium containing 2.0% sucrose (WB) supplemented
with kinetin. On WB+5.0 mg l−1 kinetin, 100% plant regeneration with 14±0.5 plantlets was obtained. Regenerated plantlets with well-developed root systems
were transferred to pots and subsequently to the field. With respect to the effect of metals on morphogenic events and quercetin
content of the cultures, lower concentrations of CuSO4 proved to be beneficial but higher concentrations were detrimental. The presence of 200 μM ZnSO4 or 150 μM CuSO4 also resulted in the highest level of quercetin in regenerants. The yield of quercetin in culture varied with stages of differentiation. |
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Keywords: | abiotic stress aminopurines Pluchea lanceolata quercetin content regeneration |
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