Human pancreatic adenocarcinoma: In vitro and in vivo morphology of a new tumor line established from ascites |
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Authors: | W H Chen J S Horoszewicz S S Leong T Shimano R Penetrante W H Sanders R Berjian H O Douglass E W Martin T M Chu |
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Institution: | (1) Present address: Cancer Institute, Chinese Academy of Medical Sciences, Beijing, People’s Republic of China;(2) Present address: Second Department of Surgery, Osaka University, School of Medicine, Osaka, Japan;(3) Roswell Park Memorial Institute, 666 Elm Street, 14263 Buffalo, NY;(4) The Ohio State University College of Medicine, Columbus, Ohio;(5) St. Luke’s Memorial Hospital Center, Utica, New York |
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Abstract: | Summary A human pancreatic tumor cell line has been established from the ascites of a patient with histopathologically confirmed adenocarcinoma
of the head of the pancreas and maintained for more than 12 months in the laboratory. Epitheloid tumor cell colonies, which
resulted from primary tissue cultures of the ascitic cell component, were mechanically isolated by needle micromanipulation.
Tumorigenicity was proven in athymic nude mice. Morphologically the pancreatic tumor epithelial cells grew to confluency with
moderately tight adhesion to the culture plastic surface and with free-floating cells in the medium. Upon re-establishment
of the tumoral xenograft in tissue culture, the epithelial cells retained their original morphology. Histologically the tumor
grown in nude mice exhibited prototypic characteristics of the primary adenocarcinoma in the patient, producing abundant mucin
and displaying a broad spectrum of glandular differentiation, which ranged from well to poorly differentiated adenocarcinomas
with occasionally localized lymphocytic infiltrations. Furthermore, the tumor expressed carcinoembryonic antigen and human
pancreas cancer associated antigen. This tumor line, designated AsPC-1, has been cultured for at least 10 passages in vitro
and 3 in vivo. It represents a new model for human pancreatic cancer.
This work was supported in part by Research Grant CA-18410 awarded by the National Cancer Institute through the National Pancreatic
Cancer Project. |
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Keywords: | ascites epithelial cells human pancreatic adenocarcinoma |
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