Occurrence of chromosomal variations and plant regeneration from long-term-cultured citrus callus |
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Authors: | Yu-Jin Hao Xiu-Xin Deng |
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Institution: | (1) National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070 Wuhan, P. R. China;(2) Present address: College of Life Sciences, Peking University, 100871 Beijing, P. R. China |
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Abstract: | Summary Embryogenic callus of Anliucheng sweet orange (Citrus sinensis Osbeck) is theoretically diploid. However, significant chromosomal variations occurred when the calluses were subcultured
and preserved for a long time. Cytological observation revealed a variety of mitotic irregularities underlying the occurrence
of chromosomal variations. Despite the ubiquitous existence of chromosomal variations, long-term-cultured calluses were still
capable of producing somatic embryos and plants. Interestingly, chromosomal variants were selected against when somatic embryos
and plants regenerated from the embryogenic callus. Randomly amplified polymorphic DNA (RAPD) analysis was also carried out
to detect DNA sequence variation in regenerated plants derived from the embryogenic callus. No difference in banding patterns
was detected. It was clear that the plant regeneration from long-term-cultured callus was inclined to select against somaclonal
variations. |
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Keywords: | mitotic irregularity somaclonal variation regeneration chromosome ploidy RAPD |
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