In vitro propagation of Spilanthes mauritiana DC., an endangered medicinal herb, through axillary bud cultures |
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Authors: | Harsh Pal Bais Julie B Green Travis S Walker Paul O Okemo Jorge M Vivanco |
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Institution: | (1) Department of Horticulture and Landscape Architecture, Colorado State University, 80523-1173 Fort Collins, CO;(2) Department of Botany, Kenyatta University, PO# 43844, Nairobi, Kenya |
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Abstract: | Summary
Spilanthes mauritiana DC., (Compositae), a East African medicinal herb containing pharmaceutically promising secondary metabolites, has successfully
been raised in vitro. We have developed a clonal propagation protocol that uses juvenile plants as starting material. The addition of benzylaminopurine
(BA) (1.0 μM) and naphthaleneacetic acid (NAA) (0.1 μM) to the culture medium resulted in maximum shooting response with minimal callusing. Shoots rooted best in vitro in MS medium supplemented with indole-3-acetic acid (IAA; 0.2 μM), and plants that had already developed roots showed better growth, with maximum survival rate, in the greenhouse after an
initial hardening. |
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Keywords: | Spilanthes mauritiana micropropagation meristem culture medicinal plant plant growth regulator |
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