In vitro micropropagation of the macarronesian evergreen treePersea indica (L.) K. Spreng |
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Authors: | Paula S Campos M Salomé S Pais |
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Institution: | 1. Departmento de Biologia Vegetal, Faculdade de Ciências da Universidade de Lisboa, Bloco C2, Campo Grande, 1700, Lisboa, Portugal
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Abstract: | Summary Shoot propagation ofPersea indica (L.) K. Spreng was achieved using seedling axillary buds cultured on MS (Murashige and Skoog, 1962) medium with 1 mg/l (2.8
μM) N6-benzyladenine (BA). Forty percent of the obtained shoots did not elongate, but showed bud proliferation, which was maximal
(three axillary buds per shoot) at the end of the seventh subculture. Sixty percent of the shoots elongated, did not show
bud proliferation, and formed calluses at their base. Successful rooting (84.6%) was achieved dipping the base of each elongated
shoot in 3 g/l (16.11 mM) indolebutyric acid (IBA) for 1–2 s, and transferring to half strength MS medium without growth regulators. These shoots
presented an acclimatization success of 100%. Results suggest that micropropagated elongated shoots ofP. indica can be adequately used in reforestation programs. |
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Keywords: | in vitro propagation macarronesian woody tree Persea indica |
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