Endothelium specific Weibel-Palade bodies in a continuous human cell line,EA.hy926 |
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Authors: | Cora-Jean S Edgell Jill E Haizlip C Robert Bagnell Joan P Packenham Paul Harrison Barry Wilbourn Victoria J Madden |
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Institution: | (1) Pathology Department 7525, University of North Carolina, 27599 Chapel Hill, North Carolina;(2) Coagulation Research, The Rayne Institute, St. Thomas' Hospital, SE1 7EH London, England |
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Abstract: | Summary Weibel-Palade bodies are ultrastructurally defined organelles found only in vascular endothelial cells. Because endothelium
in corpo is very dispersed, isolation and further characterization of this organelle has been dependent on increasing the
number of cells in culture. However, primary isolates of endothelial cells have a limited replication potential and tend to
senesce in culture. In this report, EA.hy926, a continuously replicating cell line derived from human endothelium, is shown
to contain Weibel-Palade bodies. Electron micrographs demonstrate the ultrastructural characteristics of these tissue-specific
organelles and their cytoplasmic distribution in EA.hy926 cells. Von Willebrand factor, which has been shown to exist in Weibel
Palade bodies, is demonstrated by immunofluorescence in discrete rod-shaped organelles whose size, shape, and distribution
are consistent with that of Weibel-Palade bodies in primary endothelial cell cultures. Rapid release of von Willebrand factor
can be induced by calcium ionophore, and large multimeric forms of the protein are found in EA.hy926 cells. These two properties
are consistent with the function currently ascribed to Weibel Palade bodies: storage of multimerized von Willebrand factor.
Thus ultrastructural, immunologic, and functional data establish the existence of this as yet poorly understood tissue-specific
organelle in a continuous, vigorously replicating human cell line. |
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Keywords: | Weibel-Palade bodies endothelial cells EA hy926 cells von Willebrand factor |
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