Expression and detection of endogenous mouse C-type RNA viruses |
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Authors: | Douglas R Lowy Natalie M Teich Dr Sisir K Chattopadhyay |
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Institution: | (1) Department of Dermatology, Yale University School of Medicine, New Haven, Connecticut;(2) West Haven Veterans Administration Hospital, West Haven, Connecticut;(3) Imperial Cancer Research Fund, London, England;(4) Laboratory of Viral Diseases, Building 7, Room 304, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 20014 Bethesda, Md. |
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Abstract: | Summary Many naturally occurring C-type RNA viruses are of endogenous origin. The genetic information for synthesizing these RNA viruses
is present in the DNA of normal mouse cells, probably as part of their chromosomal DNA. Some C-type viruses infect mouse cells
(homotropic virus), while others infect certain tissue culture cells from other species but not mouse fibroblasts (xenotropic
virus). All mouse strains studied appear to contain endogenous xenotropic viral genomes. However, based on the regularity
with which homotropic virus is detected, inbred mice can be divided into high, low, and nonvirus-yielding strains. Nucleic
acid hybridization studies have shown that DNA from high virus strains contains several copies of the homotropic virus genome,
while that from low virus strains contains fewer copies, and DNA from nonvirus strains lacks a significant portion of the
homotropic virus genome. In vivo and in vitro genetic studies support the nucleic acid hybridization results. In addition,
high virus mouse strains are more likely than low virus strains to release virus that will replicate efficiently in their
own cells. Methods for the activation and detection of endogenous C-type virus in tissue culture are discussed.
Presented at the Session in Depth on Endogenous Viruses in Cell Culture at the Twenty-fifth Annual Meeting of the Tissue Culture
Association, June 1974. |
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Keywords: | virus mouse virus-cell interaction endogenous virus C-type virus mouse leukemia virus |
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