Inheritance of transgenes in transgenic tall fescue (<Emphasis Type="Italic">Festuca arundinacea</Emphasis> schreb.) |
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Authors: | Email author" target="_blank">Z?Y?WangEmail author J?Bell Y?X?Ge D?Lehmann |
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Institution: | (1) Forage Biotechnology Group, The Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, 73401 Ardmore, OK |
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Abstract: | Summary Tall fescue (Festuca arundinacea Schreb.) is the most important forage species worldwide of the Festuca genus. Single genotype-derived embryogenic suspension cultures were established from tall fescue cultivar Kentucky-31, and
were used as target cells for biolistic transformation. A chimeric hygromycin phosphotransferase gene (hph) was used as the selectable marker, and a chimeric β-glucuronidase (gusA) gene was co-transformed with hph. Transgenic plants were recovered after microprojectile bombardment of suspension cells and subsequent selection in the presence
of a high concentration of hygromycin. Fertile transgenic plants were obtained after vernalization under field conditions.
T1 and T2 progenies were obtained after reciprocal crosses between transgenic and untransformed control plants. PCR and Southern
hybridization analyses revealed a 1∶1 segregation ratio for both transgenes in the T1 and T2 generations. Southern hybridization
patterns were identical for T0, T1, and T2 plants. The results demonstrated for the first time the stable meiotic transmission
of transgenes following Mendelian rules in transgenic tall fescue. |
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Keywords: | transgenic plants microprojectile bombardment biolistic transformation forage grass turf grass |
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