Amelioration of salinity tolerance in <Emphasis Type="Italic">Solanum tuberosum</Emphasis> L. by exogenous application of ascorbic acid |
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Authors: | Zahoor Ahmad Sajid Faheem Aftab |
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Institution: | (1) Department of Botany, University of the Punjab, Quaid-e-Azam Campus, Lahore, 54590, Pakistan; |
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Abstract: | The present investigation envisaged revealing the role of exogenous application of ascorbic acid in increasing resistance
against NaCl stress. Shoot apices from 60-d-old, in vitro-grown plants of two commercially important cultivars of Solanum tuberosum L., cvs. Desiree and Cardinal, were inoculated on Murashige and Skoog (MS) medium supplemented with 0.5 mM ascorbic acid
for 72 h as a pretreatment. Pretreated and non-pretreated shoot apices were transferred to MS medium containing different
concentrations of NaCl (0–140 mM; eight treatments). Results were recorded for morphological (shoot length, shoot number,
root length, root number, and number of nodes) and biochemical features (protein, peroxidase, catalase, and superoxide dismutase
activities) after 60 d of salt treatment. Similarly, 60-d-old, well-proliferated callus cultures were also pretreated with
ascorbic acid for 24 h and transferred to an optimized callus proliferation medium containing different concentrations of
salt. Results were recorded after 60 d of salt treatment for percentage relative fresh weight growth and biochemical parameters.
Salinity severely inhibited all the growth parameters in both the cultivars. Pretreatment with ascorbic acid to both salt-treated
plants and callus cultures showed significant differences with respect to almost all of the growth and biochemical parameters
studied. Protein content as well as catalase and superoxide dismutase activities increased significantly in both the cultivars,
although peroxidase activity showed a decreasing trend in ascorbic acid-pretreated plants as well as callus cultures. |
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